• Login
    View Item 
    •   Home
    • Research
    • Articles
    • View Item
    •   Home
    • Research
    • Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguideTheses and Dissertations LibguideSubmit an Item

    Statistics

    Display statistics

    Rice calcium-dependent protein kinase OsCPK17 targets plasma membrane intrinsic protein and sucrose phosphate synthase and is required for a proper cold stress response

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Almadanim_et_al-2017.pdf
    Size:
    1.255Mb
    Format:
    PDF
    Description:
    Accepted Manuscript
    Download
    Thumbnail
    Name:
    c6cb513be9f6bc38761ca30326a4c399c954bcaa4aad6a7ddd241a2d3a1a1b60.sup-1.pdf
    Size:
    1.075Mb
    Format:
    PDF
    Description:
    Supplemental files
    Download
    Type
    Article
    Authors
    Almadanim, M. Cecília
    Alexandre, Bruno M.
    Rosa, Margarida T.G.
    Sapeta, Helena
    Leitão, António E.
    Ramalho, José C.
    Lam, TuKiet T.
    Negrão, Sónia cc
    Abreu, Isabel A. cc
    Oliveira, M. Margarida
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Date
    2017-03-02
    Online Publication Date
    2017-03-02
    Print Publication Date
    2017-07
    Permanent link to this record
    http://hdl.handle.net/10754/622723
    
    Metadata
    Show full item record
    Abstract
    Calcium-dependent protein kinases (CDPKs) are involved in plant tolerance mechanisms to abiotic stresses. Although CDPKs are recognized as key messengers in signal transduction, the specific role of most members of this family remains unknown. Here we test the hypothesis that OsCPK17 plays a role in rice cold stress response by analyzing OsCPK17 knockout, silencing, and overexpressing rice lines under low temperature. Altered OsCPK17 gene expression compromises cold tolerance performance, without affecting the expression of key cold stress-inducible genes. A comparative phosphoproteomic approach led to the identification of six potential in vivo OsCPK17 targets, which are associated with sugar and nitrogen metabolism, and with osmotic regulation. To test direct interaction, in vitro kinase assays were performed, showing that the sucrose phosphate synthase OsSPS4, and the aquaporin OsPIP2;1/OsPIP2;6 are phosphorylated by OsCPK17 in a calcium-dependent manner. Altogether, our data indicates that OsCPK17 is required for a proper cold stress response in rice, likely affecting the activity of membrane channels and sugar metabolism.
    Citation
    Almadanim MC, Alexandre BM, Rosa MTG, Sapeta H, Leitão AE, et al. (2017) Rice calcium-dependent protein kinase OsCPK17 targets plasma membrane intrinsic protein and sucrose phosphate synthase and is required for a proper cold stress response. Plant, Cell & Environment. Available: http://dx.doi.org/10.1111/pce.12916.
    Sponsors
    We acknowledge: Alexandra Marques for cloning OsCPK17 into pDEST28; Pedro Pereira for the help with the transgenic rice plants; Nuno Gonçalves, Mafalda Rodrigues and Vanessa Azevedo for the aid with electrolyte leakage datapoint sample collection; Tiago Lourenço and Ana Paula Farinha for providing some of the primers used; Margarida Saramago for the support with scintillation counter equipment and borrowed material; Edward Voss, Jean Kanyo, and Kathrin Wilczak for their assistance in MS sample preparation, data collection, and initial data analysis, respectively. We also thank Rossana Henriques (CRAG), Nelson Saibo (ITQB), and Alison Funston (Monash University, Australia) for critical revision. This research was funded by Portuguese Fundação para a Ciência e a Tecnologia: grants SFRH/BD/61121/2009 to M.C.A., SFRH/BD/84219/2012 to M.T.G.R., SFRH/BD/89781/2012 to H.S., SFRH/BPD/98619/2013 to B.M.A.; FCT Investigator (POPH-QREN) to I.A.A.. The work was also supported by the FCT research units GREEN-it
    Publisher
    Wiley
    Journal
    Plant, Cell & Environment
    DOI
    10.1111/pce.12916
    Additional Links
    http://onlinelibrary.wiley.com/doi/10.1111/pce.12916/abstract
    ae974a485f413a2113503eed53cd6c53
    10.1111/pce.12916
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division

    entitlement

     
    DSpace software copyright © 2002-2022  DuraSpace
    Quick Guide | Contact Us | KAUST University Library
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.