Show simple item record

dc.contributor.authorBarbato, Marta
dc.contributor.authorScoma, Alberto
dc.contributor.authorMapelli, Francesca
dc.contributor.authorDe Smet, Rebecca
dc.contributor.authorBanat, Ibrahim M.
dc.contributor.authorDaffonchio, Daniele
dc.contributor.authorBoon, Nico
dc.contributor.authorBorin, Sara
dc.date.accessioned2017-01-15T13:40:35Z
dc.date.available2017-01-15T13:40:35Z
dc.date.issued2016-12-21
dc.identifier.citationBarbato M, Scoma A, Mapelli F, De Smet R, Banat IM, et al. (2016) Hydrocarbonoclastic Alcanivorax Isolates Exhibit Different Physiological and Expression Responses to n-dodecane. Frontiers in Microbiology 7. Available: http://dx.doi.org/10.3389/fmicb.2016.02056.
dc.identifier.issn1664-302X
dc.identifier.doi10.3389/fmicb.2016.02056
dc.identifier.urihttp://hdl.handle.net/10754/622690
dc.description.abstractAutochthonous microorganisms inhabiting hydrocarbon polluted marine environments play a fundamental role in natural attenuation and constitute promising resources for bioremediation approaches. Alcanivorax spp. members are ubiquitous in contaminated surface waters and are the first to flourish on a wide range of alkanes after an oil-spill. Following oil contamination, a transient community of different Alcanivorax spp. develop, but whether they use a similar physiological, cellular and transcriptomic response to hydrocarbon substrates is unknown. In order to identify which cellular mechanisms are implicated in alkane degradation, we investigated the response of two isolates belonging to different Alcanivorax species, A. dieselolei KS 293 and A. borkumensis SK2 growing on n-dodecane (C12) or on pyruvate. Both strains were equally able to grow on C12 but they activated different strategies to exploit it as carbon and energy source. The membrane morphology and hydrophobicity of SK2 changed remarkably, from neat and hydrophilic on pyruvate to indented and hydrophobic on C12, while no changes were observed in KS 293. In addition, SK2 accumulated a massive amount of intracellular grains when growing on pyruvate, which might constitute a carbon reservoir. Furthermore, SK2 significantly decreased medium surface tension with respect to KS 293 when growing on C12, as a putative result of higher production of biosurfactants. The transcriptomic responses of the two isolates were also highly different. KS 293 changes were relatively balanced when growing on C12 with respect to pyruvate, giving almost the same amount of upregulated (28%), downregulated (37%) and equally regulated (36%) genes, while SK2 transcription was upregulated for most of the genes (81%) when growing on pyruvate when compared to C12. While both strains, having similar genomic background in genes related to hydrocarbon metabolism, retained the same capability to grow on C12, they nevertheless presented very different physiological, cellular and transcriptomic landscapes.
dc.description.sponsorshipThis work was funded by FP-7 project Kill Spill (No. 312139,
dc.publisherFrontiers Media SA
dc.relation.urlhttp://journal.frontiersin.org/article/10.3389/fmicb.2016.02056/full
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAlcanivorax
dc.subjecttranscriptomics
dc.subjectalkanes
dc.subjectbioremediation
dc.subjectfunctional redundancy
dc.titleHydrocarbonoclastic Alcanivorax Isolates Exhibit Different Physiological and Expression Responses to n-dodecane
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalFrontiers in Microbiology
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionCentre for Microbial Ecology and Technology, Ghent University, Ghent, Belgium
dc.contributor.institutionDepartment of Food, Environmental and Nutritional Sciences, University of Milan, Milan, Italy
dc.contributor.institutionDepartment of Medical and Forensic Pathology, University of Ghent, Ghent, Belgium
dc.contributor.institutionSchool of Biomedical Sciences, University of Ulster, Coleraine, UK
kaust.personDaffonchio, Daniele
refterms.dateFOA2018-06-13T12:51:23Z


Files in this item

Thumbnail
Name:
fmicb-07-02056.pdf
Size:
2.490Mb
Format:
PDF
Description:
Main article
Thumbnail
Name:
presentation 1.pptx
Size:
58.60Kb
Format:
Microsoft PowerPoint 2007
Description:
Supplemental files
Thumbnail
Name:
table 1.xls
Size:
233.5Kb
Format:
Microsoft Excel
Description:
Supplemental files

This item appears in the following Collection(s)

Show simple item record

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.