A study of the incubation of microbead agglutination assays in a microfluidic system
KAUST DepartmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Electrical Engineering Program
Physical Sciences and Engineering (PSE) Division
Mechanical Engineering Program
Permanent link to this recordhttp://hdl.handle.net/10754/622509
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AbstractThis work reports on a quantitative study of the incubation of a microbead-based agglutination assay inside a microfluidic system. In this system, a droplet (1.25µL) consisting of a mixture of functionalized microbeads and analyte is flowed through a 0.51mm internal diameter silicone tube. Hydrodynamic forces alone produce a very efficient mixing of the beads within the droplet. We tested the agglutination at different speeds and show a robust response at the higher range of speeds (150 – 200µL/min), while also reaching a completion in the agglutination process. At these velocities, a length of 180cm is shown to be sufficient to confidently measure the agglutination assay, which takes between 2.5 – 3 minutes. This high throughput quantification method has the potential of accelerating the measurements of various types of biomarkers, which can greatly benefit the fields of biology and medicine.
CitationCastro D, Conchouso D, Arevalo A, Foulds IG (2016) A study of the incubation of microbead agglutination assays in a microfluidic system. 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). Available: http://dx.doi.org/10.1109/NEMS.2016.7758266.
Journal2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)