Isolation and characterization of 29 microsatellite markers for the bumphead parrotfish, Bolbometopon muricatum, and cross amplification in 12 related species
Almany, Glenn R.
Braun, Camrin D.
Hamilton, Richard J.
Saenz Agudelo, Pablo
Berumen, Michael L.
KAUST DepartmentRed Sea Research Center (RSRC)
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AbstractWe isolated and characterized 29 microsatellite loci for the bumphead parrotfish, Bolbometopon muricatum, a wide-ranging parrotfish listed as vulnerable by the International Union for Conservation of Nature (IUCN). The 29 loci were tested on 95 individuals sampled from the Solomon Islands. The number of alleles ranged from two to ten. Evidence of linkage disequilibrium was found for only one pair of loci (Bm54 and Bm112). Two loci (Bm20 and Bm119) showed significant departure from Hardy-Weinberg equilibrium. We also tested each locus for amplification and polymorphism on 11 other scarine labrid species and one labrid species. Amplification success ranged from zero to ten loci per species. These microsatellite loci are the first specific set for B. muricatum and will be a useful tool for assessing genetic population structure, genetic diversity, and parentage in future studies.
CitationPriest MA, Almany GR, Braun CD, Hamilton RJ, Lozano-Cortés DF, et al. (2014) Isolation and characterization of 29 microsatellite markers for the bumphead parrotfish, Bolbometopon muricatum, and cross amplification in 12 related species. Marine Biodiversity 45: 861–866. Available: http://dx.doi.org/10.1007/s12526-014-0278-4.
SponsorsThe authors wish to thank the Biosciences Core Lab of the King Abdullah University of Science and Technology (KAUST) for technical assistance, Hugo Harrison for the repurposing of Fig. 1, and three anonymous reviewers for helpful comments. Funding was provided in part by KAUST through baseline research funding and through award number CRG-1-BER-002 to MLB.
PublisherSpringer Science + Business Media