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dc.contributor.authorMarquet, P.
dc.contributor.authorDepeursinge, Christian
dc.contributor.authorJourdain, P.
dc.date.accessioned2017-01-02T08:42:36Z
dc.date.available2017-01-02T08:42:36Z
dc.date.issued2016-11-22
dc.identifier.citationMarquet P, Depeursinge C, Jourdain P (2017) Cellular Dynamics Revealed by Digital Holographic Microscopy☆. Reference Module in Neuroscience and Biobehavioral Psychology. Available: http://dx.doi.org/10.1016/B978-0-12-809324-5.02426-3.
dc.identifier.doi10.1016/B978-0-12-809324-5.02426-3
dc.identifier.urihttp://hdl.handle.net/10754/622179
dc.description.abstractDigital holographic microscopy (DHM) is a new optical method that provides, without the use of any contrast agent, real-time, three-dimensional images of transparent living cells, with an axial sensitivity of a few tens of nanometers. They result from the hologram numerical reconstruction process, which permits a sub wavelength calculation of the phase shift, produced on the transmitted wave front, by the optically probed cells, namely the quantitative phase signal (QPS). Specifically, in addition to measurements of cellular surface morphometry and intracellular refractive index (RI), various biophysical cellular parameters including dry mass, absolute volume, membrane fluctuations at the nanoscale and biomechanical properties, transmembrane water permeability as swell as current, can be derived from the QPS. This article presents how quantitative phase DHM (QP-DHM) can explored cell dynamics at the nanoscale with a special attention to both the study of neuronal dynamics and the optical resolution of local neuronal network.
dc.publisherElsevier BV
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/B9780128093245024263
dc.subjectCellular dynamics
dc.subjectCotransporters
dc.subjectDigital holographic microscopy
dc.subjectExcitotoxicity
dc.subjectGlutamate
dc.subjectInterferometry
dc.subjectMicroscopy
dc.subjectNeuron cell culture
dc.subjectOptical imaging
dc.subjectTransmembrane water movements
dc.titleCellular Dynamics Revealed by Digital Holographic Microscopy☆
dc.typeBook Chapter
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalReference Module in Neuroscience and Biobehavioral Psychology
dc.contributor.institutionUniversitaire Vaudois, Prilly/Lausanne, Switzerland
dc.contributor.institutionInstitut Universitaire en Santé Mentale de Québec, Quebec, QC, Canada
dc.contributor.institutionUniversité Laval, Quebec, QC, Canada
dc.contributor.institutionBrain Mind Institute, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
kaust.personDepeursinge, Christian
dc.date.published-online2016-11-22
dc.date.published-print2017


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