Acquisition and Analysis of Data from High Concentration Solutions
dc.contributor.author | Besong, Tabot M.D. | |
dc.contributor.author | Rowe, Arthur J. | |
dc.date.accessioned | 2017-01-02T08:42:35Z | |
dc.date.available | 2017-01-02T08:42:35Z | |
dc.date.issued | 2016-05-14 | |
dc.identifier.citation | Besong TMD, Rowe AJ (2016) Acquisition and Analysis of Data from High Concentration Solutions. Analytical Ultracentrifugation: 499–520. Available: http://dx.doi.org/10.1007/978-4-431-55985-6_25. | |
dc.identifier.doi | 10.1007/978-4-431-55985-6_25 | |
dc.identifier.uri | http://hdl.handle.net/10754/622170 | |
dc.description.abstract | The problems associated with ultracentrifugal analysis of macromolecular solutions at high (>10 mg/ml) are reviewed. Especially for the case of solutes which are non-monodisperse, meaningful results are not readily achievable using sedimentation velocity approaches. It is shown however by both simulation and analysis of practical data that using a modified form of an algorithm (INVEQ) published in other contexts, sedimentation equilibrium (SE) profiles can be analysed successfully, enabling topics such as oligomer presence or formation to be defined.To achieve this, it is necessary to employ an approach in which the solution density, which in an SE profile is radius-dependent, is taken into consideration. Simulation suggests that any reasonable level of solute concentration can be analysed. | |
dc.publisher | Springer Nature | |
dc.subject | Analytical ultracentrifuge | |
dc.subject | Concentrated solutions | |
dc.subject | Oligomers | |
dc.subject | Sedimentation velocity | |
dc.title | Acquisition and Analysis of Data from High Concentration Solutions | |
dc.type | Book Chapter | |
dc.contributor.department | Functional Nanomaterials Lab (FuNL) | |
dc.contributor.department | KAUST Solar Center (KSC) | |
dc.contributor.department | Physical Science and Engineering (PSE) Division | |
dc.identifier.journal | Analytical Ultracentrifugation | |
dc.contributor.institution | NCMH School of Biosciences, University of Nottingham, Sutton Bonington, United Kingdom | |
kaust.person | Besong, Tabot M.D. | |
dc.date.published-online | 2016-05-14 | |
dc.date.published-print | 2016 |
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Physical Science and Engineering (PSE) Division
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Book Chapters
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KAUST Solar Center (KSC)