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dc.contributor.authorBesong, Tabot M.D.
dc.contributor.authorRowe, Arthur J.
dc.date.accessioned2017-01-02T08:42:35Z
dc.date.available2017-01-02T08:42:35Z
dc.date.issued2016-05-14
dc.identifier.citationBesong TMD, Rowe AJ (2016) Acquisition and Analysis of Data from High Concentration Solutions. Analytical Ultracentrifugation: 499–520. Available: http://dx.doi.org/10.1007/978-4-431-55985-6_25.
dc.identifier.doi10.1007/978-4-431-55985-6_25
dc.identifier.urihttp://hdl.handle.net/10754/622170
dc.description.abstractThe problems associated with ultracentrifugal analysis of macromolecular solutions at high (>10 mg/ml) are reviewed. Especially for the case of solutes which are non-monodisperse, meaningful results are not readily achievable using sedimentation velocity approaches. It is shown however by both simulation and analysis of practical data that using a modified form of an algorithm (INVEQ) published in other contexts, sedimentation equilibrium (SE) profiles can be analysed successfully, enabling topics such as oligomer presence or formation to be defined.To achieve this, it is necessary to employ an approach in which the solution density, which in an SE profile is radius-dependent, is taken into consideration. Simulation suggests that any reasonable level of solute concentration can be analysed.
dc.publisherSpringer Nature
dc.subjectAnalytical ultracentrifuge
dc.subjectConcentrated solutions
dc.subjectOligomers
dc.subjectSedimentation velocity
dc.titleAcquisition and Analysis of Data from High Concentration Solutions
dc.typeBook Chapter
dc.contributor.departmentFunctional Nanomaterials Lab (FuNL)
dc.contributor.departmentKAUST Solar Center (KSC)
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalAnalytical Ultracentrifugation
dc.contributor.institutionNCMH School of Biosciences, University of Nottingham, Sutton Bonington, United Kingdom
kaust.personBesong, Tabot M.D.
dc.date.published-online2016-05-14
dc.date.published-print2016


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