Acquisition and Analysis of Data from High Concentration Solutions
Type
Book ChapterAuthors
Besong, Tabot M.D.
Rowe, Arthur J.
KAUST Department
Functional Nanomaterials Lab (FuNL)KAUST Solar Center (KSC)
Physical Science and Engineering (PSE) Division
Date
2016-05-14Online Publication Date
2016-05-14Print Publication Date
2016Permanent link to this record
http://hdl.handle.net/10754/622170
Metadata
Show full item recordAbstract
The problems associated with ultracentrifugal analysis of macromolecular solutions at high (>10 mg/ml) are reviewed. Especially for the case of solutes which are non-monodisperse, meaningful results are not readily achievable using sedimentation velocity approaches. It is shown however by both simulation and analysis of practical data that using a modified form of an algorithm (INVEQ) published in other contexts, sedimentation equilibrium (SE) profiles can be analysed successfully, enabling topics such as oligomer presence or formation to be defined.To achieve this, it is necessary to employ an approach in which the solution density, which in an SE profile is radius-dependent, is taken into consideration. Simulation suggests that any reasonable level of solute concentration can be analysed.Citation
Besong TMD, Rowe AJ (2016) Acquisition and Analysis of Data from High Concentration Solutions. Analytical Ultracentrifugation: 499–520. Available: http://dx.doi.org/10.1007/978-4-431-55985-6_25.Publisher
Springer NatureJournal
Analytical Ultracentrifugationae974a485f413a2113503eed53cd6c53
10.1007/978-4-431-55985-6_25