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dc.contributor.authorAli, Shawkat*
dc.contributor.authorBakkeren, Guus*
dc.date.accessioned2017-01-02T08:10:21Z
dc.date.available2017-01-02T08:10:21Z
dc.date.issued2014-09-19en
dc.identifier.citationAli S, Bakkeren G (2014) Conversion of BAC Clones into Binary BAC (BIBAC) Vectors and Their Delivery into Basidiomycete Fungal Cells Using Agrobacterium tumefaciens. Bacterial Artificial Chromosomes: 199–215. Available: http://dx.doi.org/10.1007/978-1-4939-1652-8_9.en
dc.identifier.issn1064-3745en
dc.identifier.issn1940-6029en
dc.identifier.doi10.1007/978-1-4939-1652-8_9en
dc.identifier.urihttp://hdl.handle.net/10754/622139
dc.description.abstractThe genetic transformation of certain organisms, required for gene function analysis or complementation, is often not very efficient, especially when dealing with large gene constructs or genomic fragments. We have adapted the natural DNA transfer mechanism from the soil pathogenic bacterium Agrobacterium tumefaciens, to deliver intact large DNA constructs to basidiomycete fungi of the genus Ustilago where they stably integrated into their genome. To this end, Bacterial Artificial Chromosome (BAC) clones containing large fungal genomic DNA fragments were converted via a Lambda phage-based recombineering step to Agrobacterium transfer-competent binary vectors (BIBACs) with a Ustilago-specific selection marker. The fungal genomic DNA fragment was subsequently successfully delivered as T-DNA through Agrobacterium-mediated transformation into Ustilago species where an intact copy stably integrated into the genome. By modifying the recombineering vector, this method can theoretically be adapted for many different fungi.en
dc.publisherSpringer Science + Business Mediaen
dc.subjectAgrobacterium-mediated transformationen
dc.subjectBacterial artificial chromosomeen
dc.subjectBasidiomyceteen
dc.subjectBinary vectoren
dc.subjectRecombineeringen
dc.subjectUstilagoen
dc.titleConversion of BAC Clones into Binary BAC (BIBAC) Vectors and Their Delivery into Basidiomycete Fungal Cells Using Agrobacterium tumefaciensen
dc.typeBook Chapteren
dc.contributor.departmentDesert Agriculture Initiative*
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division*
dc.identifier.journalBacterial Artificial Chromosomesen
dc.contributor.institutionAgriculture and Agri-Food Canada, Pacific Agri-Food Research Centre, Summerland, BC, Canada*
dc.contributor.institutionDepartment of Botany, University of British Columbia, Vancouver, BC, Canada*
kaust.authorAli, Shawkat*


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