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dc.contributor.authorAli, Shawkat
dc.contributor.authorBakkeren, Guus
dc.date.accessioned2017-01-02T08:10:21Z
dc.date.available2017-01-02T08:10:21Z
dc.date.issued2014-09-03
dc.identifier.citationAli S, Bakkeren G (2014) Conversion of BAC Clones into Binary BAC (BIBAC) Vectors and Their Delivery into Basidiomycete Fungal Cells Using Agrobacterium tumefaciens. Bacterial Artificial Chromosomes: 199–215. Available: http://dx.doi.org/10.1007/978-1-4939-1652-8_9.
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.doi10.1007/978-1-4939-1652-8_9
dc.identifier.urihttp://hdl.handle.net/10754/622139
dc.description.abstractThe genetic transformation of certain organisms, required for gene function analysis or complementation, is often not very efficient, especially when dealing with large gene constructs or genomic fragments. We have adapted the natural DNA transfer mechanism from the soil pathogenic bacterium Agrobacterium tumefaciens, to deliver intact large DNA constructs to basidiomycete fungi of the genus Ustilago where they stably integrated into their genome. To this end, Bacterial Artificial Chromosome (BAC) clones containing large fungal genomic DNA fragments were converted via a Lambda phage-based recombineering step to Agrobacterium transfer-competent binary vectors (BIBACs) with a Ustilago-specific selection marker. The fungal genomic DNA fragment was subsequently successfully delivered as T-DNA through Agrobacterium-mediated transformation into Ustilago species where an intact copy stably integrated into the genome. By modifying the recombineering vector, this method can theoretically be adapted for many different fungi.
dc.publisherSpringer Nature
dc.subjectAgrobacterium-mediated transformation
dc.subjectBacterial artificial chromosome
dc.subjectBasidiomycete
dc.subjectBinary vector
dc.subjectRecombineering
dc.subjectUstilago
dc.titleConversion of BAC Clones into Binary BAC (BIBAC) Vectors and Their Delivery into Basidiomycete Fungal Cells Using Agrobacterium tumefaciens
dc.typeBook Chapter
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentCenter for Desert Agriculture
dc.identifier.journalBacterial Artificial Chromosomes
dc.contributor.institutionAgriculture and Agri-Food Canada, Pacific Agri-Food Research Centre, Summerland, BC, Canada
dc.contributor.institutionDepartment of Botany, University of British Columbia, Vancouver, BC, Canada
kaust.personAli, Shawkat
dc.date.published-online2014-09-03
dc.date.published-print2015


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