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dc.contributor.authorBattista, Edmondo
dc.contributor.authorColuccio, Maria Laura
dc.contributor.authorAlabastri, Alessandro
dc.contributor.authorBarberio, Marianna
dc.contributor.authorCausa, Filippo
dc.contributor.authorNetti, Paolo Antonio
dc.contributor.authorDi Fabrizio, Enzo M.
dc.contributor.authorGentile, Francesco
dc.date.accessioned2016-12-20T05:52:11Z
dc.date.available2016-12-20T05:52:11Z
dc.date.issued2016-12-15
dc.identifier.citationBattista E, Coluccio ML, Alabastri A, Barberio M, Causa F, et al. (2016) Metal Enhanced Fluorescence on Super-Hydrophobic Clusters of Gold Nanoparticles. Microelectronic Engineering. Available: http://dx.doi.org/10.1016/j.mee.2016.12.013.
dc.identifier.issn0167-9317
dc.identifier.doi10.1016/j.mee.2016.12.013
dc.identifier.urihttp://hdl.handle.net/10754/622043
dc.description.abstractWe used optical lithography, electroless deposition and deep reactive ion etching techniques to realize arrays of super-hydrophobic gold nanoparticles arranged in a hierarchical structure. At the micro-scale, silicon-micro pillars in the chip permit to manipulate and concentrate biological solutions, at the nano-scale, gold nanoparticles enable metal enhanced fluorescence (MEF) effects, whereby fluorescence signal of fluorophores in close proximity to a rough metal surface is amplified by orders of magnitude. Here, we demonstrated the device in the analysis of fluorescein derived gold-binding peptides (GBP-FITC). While super-hydrophobic schemes and MEF effects have been heretofore used in isolation, their integration in a platform may advance the current state of fluorescence-based sensing technology in medical diagnostics and biotechnology. This scheme may be employed in protein microarrays where the increased sensitivity of the device may enable the early detection of cancer biomarkers or other proteins of biomedical interest.
dc.description.sponsorshipThis work has been partially funded from the Italian Minister of Health (Project n. GR-2010-2320665) and by UE, MIUR and Ministero dello Sviluppo Economico (project ITEM (Infrastruttura per Tecnologie BioMEMS di Sensing Avanzato per Monitoraggio e Diagnostica Ambientale e Alimentare) PONa3_00077).
dc.publisherElsevier BV
dc.relation.urlhttp://www.sciencedirect.com/science/article/pii/S0167931716305184
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Microelectronic Engineering. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Microelectronic Engineering, 15 December 2016. DOI: 10.1016/j.mee.2016.12.013
dc.subjectMetal Enhanced Fluorescence (MEF)
dc.subjectSuper-Hydrophobic Surfaces
dc.subjectGold Nanoparticles
dc.subjectElectroless Deposition
dc.subjectGold-binding Peptides
dc.subjectFluorescence Lifetime Microscopy (FLIM)
dc.titleMetal Enhanced Fluorescence on Super-Hydrophobic Clusters of Gold Nanoparticles
dc.typeArticle
dc.contributor.departmentMaterial Science and Engineering Program
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalMicroelectronic Engineering
dc.eprint.versionPost-print
dc.contributor.institutionInterdisciplinary Research Center on Biomaterials and DICMAPI, University Federico II, P.le Tecchio 80, Naples, Italy
dc.contributor.institutionCenter for Advanced Biomaterials for HealthCare @CRIB, Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, Naples, Italy
dc.contributor.institutionDepartment of Experimental and Clinical Medicine, University of Magna Graecia, 88100 Catanzaro, Italy
dc.contributor.institutionPhysics and Astronomy Department, Rice University, Houston, Tx, USA
dc.contributor.institutionIpat-lab, INRS-EMT, 1650 Boul Lionel Boulet, Varennes, (Qb), Canada
dc.contributor.institutionDepartment of Electrical Engineering and Information Technology, University Federico II, Naples, Italy
kaust.personDi Fabrizio, Enzo M.
dc.date.published-online2016-12-15
dc.date.published-print2017-05


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