Metal Enhanced Fluorescence on Super-Hydrophobic Clusters of Gold Nanoparticles
Coluccio, Maria Laura
Netti, Paolo Antonio
Di Fabrizio, Enzo M.
KAUST DepartmentPhysical Sciences and Engineering (PSE) Division
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AbstractWe used optical lithography, electroless deposition and deep reactive ion etching techniques to realize arrays of super-hydrophobic gold nanoparticles arranged in a hierarchical structure. At the micro-scale, silicon-micro pillars in the chip permit to manipulate and concentrate biological solutions, at the nano-scale, gold nanoparticles enable metal enhanced fluorescence (MEF) effects, whereby fluorescence signal of fluorophores in close proximity to a rough metal surface is amplified by orders of magnitude. Here, we demonstrated the device in the analysis of fluorescein derived gold-binding peptides (GBP-FITC). While super-hydrophobic schemes and MEF effects have been heretofore used in isolation, their integration in a platform may advance the current state of fluorescence-based sensing technology in medical diagnostics and biotechnology. This scheme may be employed in protein microarrays where the increased sensitivity of the device may enable the early detection of cancer biomarkers or other proteins of biomedical interest.
CitationBattista E, Coluccio ML, Alabastri A, Barberio M, Causa F, et al. (2016) Metal Enhanced Fluorescence on Super-Hydrophobic Clusters of Gold Nanoparticles. Microelectronic Engineering. Available: http://dx.doi.org/10.1016/j.mee.2016.12.013.
SponsorsThis work has been partially funded from the Italian Minister of Health (Project n. GR-2010-2320665) and by UE, MIUR and Ministero dello Sviluppo Economico (project ITEM (Infrastruttura per Tecnologie BioMEMS di Sensing Avanzato per Monitoraggio e Diagnostica Ambientale e Alimentare) PONa3_00077).