Show simple item record

dc.contributor.authorDiaz Rua, Ruben
dc.contributor.authorPalou, Andreu
dc.contributor.authorOliver, Paula
dc.date.accessioned2016-12-18T13:36:59Z
dc.date.available2016-12-18T13:36:59Z
dc.date.issued2016-11-23
dc.identifier.citationDíaz-Rúa R, Palou A, Oliver P (2016) Cpt1a gene expression in peripheral blood mononuclear cells as an early biomarker of diet-related metabolic alterations. Food & Nutrition Research 60. Available: http://dx.doi.org/10.3402/fnr.v60.33554.
dc.identifier.issn1654-661X
dc.identifier.issn1654-6628
dc.identifier.doi10.3402/fnr.v60.33554
dc.identifier.urihttp://hdl.handle.net/10754/622030
dc.description.abstractBackground: Research on biomarkers that provide early information about the development of future metabolic alterations is an emerging discipline. Gene expression analysis in peripheral blood mononuclear cells (PBMC) is a promising tool to identify subjects at risk of developing diet-related diseases.
dc.description.abstractObjective: We analysed PBMC expression of key energy homeostasis-related genes in a time-course analysis in order to find out early markers of metabolic alterations due to sustained intake of high-fat (HF) and highprotein (HP) diets.
dc.description.abstractDesign: We administered HF and HP diets (4 months) to adult Wistar rats in isocaloric conditions to a control diet, mainly to avoid overweight associated with the intake of hyperlipidic diets and, thus, to be able to characterise markers of metabolically obese normal-weight (MONW) syndrome. PBMC samples were collected at different time points of dietary treatment and expression of relevant energy homeostatic genes analysed by real-time reverse transcription-polymerase chain reaction. Serum parameters related with metabolic syndrome, as well as fat deposition in liver, were also analysed.
dc.description.abstractResults: The most outstanding results were those obtained for the expression of the lipolytic gene carnitine palmitoyltransferase 1a (Cpt1a). Cpt1a expression in PBMC increased after only 1 month of exposure to both unbalanced diets, and this increased expression was maintained thereafter. Interestingly, in the case of the HF diet, Cpt1a expression was altered even in the absence of increased body weight but correlated with alterations such as higher insulin resistance, alteration of serum lipid profile and, particularly, increased fat deposition in liver, a feature characteristic of metabolic syndrome, which was even observed in animals fed with HP diet.
dc.description.abstractConclusions: We propose Cpt1a gene expression analysis in PBMC as an early biomarker of metabolic alterations associated with MONW phenotype due to the intake of isocaloric HF diets, as well as a marker of increased risk of metabolic diseases associated with the intake of HF or HP diets.
dc.description.sponsorshipCIBER de Fisiopatologia de la Obesidad y Nutricion is an initiative of the Instituto de Salud Carlos III (ISCIII). This work was supported by the Spanish Government (Ministerio de Economia y Competitividad, INTERBIOBES, AGL2015-67019-P) and by the University of the Balearic Islands (IBIFLEX, FA38/2016). The Laboratory of Molecular Biology, Nutrition and Biotechnology is a member of the European Research Network of Excellence NuGO (The European Nutrigenomics Organisation). RDR was recipient of a fellowship from the Spanish Government.
dc.publisherSNF Swedish Nutrition Foundation
dc.relation.urlhttp://www.foodandnutritionresearch.net/index.php/fnr/article/view/33554
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectdietary markers
dc.subjectprevention markers
dc.subjectPBMC
dc.subjecthigh-fat diet
dc.subjecthigh-protein diet
dc.subjectunbalanced diets
dc.subjectmetabolic syndrome
dc.subjectliver steatosis
dc.titleCpt1a gene expression in peripheral blood mononuclear cells as an early biomarker of diet-related metabolic alterations
dc.typeArticle
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.identifier.journalFood & Nutrition Research
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionLaboratory of Molecular Biology, Nutrition and Biotechnology, Universitat de les Illes Balears and CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), Palma de Mallorca, Spain
kaust.personDiaz Rua, Ruben
refterms.dateFOA2018-06-13T14:44:18Z
dc.date.published-online2016-11-23
dc.date.published-print2016-01


Files in this item

Thumbnail
Name:
33554-218428-1-PB.pdf
Size:
772.0Kb
Format:
PDF
Description:
Main article
Thumbnail
Name:
33554-218466-1-SP.docx
Size:
32.29Kb
Format:
Microsoft Word 2007
Description:
Supplemental files

This item appears in the following Collection(s)

Show simple item record

This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.
Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.