• Login
    View Item 
    •   Home
    • Theses and Dissertations
    • PhD Dissertations
    • View Item
    •   Home
    • Theses and Dissertations
    • PhD Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguideTheses and Dissertations LibguideSubmit an Item

    Statistics

    Display statistics

    Bio-prospecting of Plants and Marine Organisms in Saudi Arabia for New Potential Bioactivity

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Dina Hajjar - Dissertation - Final Draft.pdf
    Size:
    3.146Mb
    Format:
    PDF
    Download
    Type
    Dissertation
    Authors
    Hajjar, Dina A. cc
    Advisors
    Ravasi, Timothy cc
    Committee members
    Voolstra, Christian R. cc
    Pinnau, Ingo cc
    Hentschel Humeida, Ute
    Program
    Chemical and Biological Engineering
    KAUST Department
    Physical Science and Engineering (PSE) Division
    Date
    2016-12-08
    Embargo End Date
    2017-12-11
    Permanent link to this record
    http://hdl.handle.net/10754/621995
    
    Metadata
    Show full item record
    Access Restrictions
    At the time of archiving, the student author of this dissertation opted to temporarily restrict access to it. The full text of this dissertation became available to the public after the expiration of the embargo on 2017-12-11.
    Abstract
    The natural resources offer a unique opportunity for the discovery of active compounds, due to the complexity and biodiversity of their chemical structures. Natural resources have been used as medicines throughout human history. Saudi Arabia’s natural resources, for instance its terrestrial medicinal plants and the Red Sea sponges, have not been extensively investigated with regard to their biological activities. To better identify the diversity of compounds with bioactive potential, new techniques are also necessary in order to improve the drug discovery path. This study comprises three sections. The first section examines Juniperus phoenicea (Arar), Anastatica hierochuntica (Kaff Maryam) and Citrullus colocynthis (Hanzal); these herbal plants were screened for potential bioactivity using a newly developed pipeline based on a high-content screening technique. We report a new cell-based high-throughput phenotypic screening for the bio-prospecting of unknown natural products from Saudi Arabian plants, in order to reveal their biological activities. The second section investigates Avicennia marina plants, screened for reverse transcriptase anti-HIV bioactivity using biochemical assay. Image-based high-content screening with a set of cellular stains was used to investigate the phenotypic results of toxicity and cell cycle arrest. The third section considers the isolation of Actinomycetes from Red Sea Sponges. Actinomycetes bacterial isolates were tested for bioactivity against West Nile Virus NS3 Protease. Analytical chemical techniques such as liquid chromatography–mass spectrometry (LC-MS), gas chromatography–mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) were used to gain more understanding of the possible chemical compounds responsible for this bioactivity. Overall, the aim of this work is to investigate the potential bioactive effect of several Saudi Arabian plants and Red Sea sponges against cancer cells and viral infections. Our study demonstrates the efficiency of the newly developed pipeline using cell-based phenotypic screening. Anti-cancer potential activity was detected in Juniperus phoenicea. Bioactive potential against the reverse transcriptase enzyme of HIV virus was confirmed in Avicennia marina leaves. The organic extracts of Actinomycetes bacterial isolates were found active against West Nile Virus NS3 Protease. Here, promising starting point for the potential of drug discovery of plants and marine organism of Saudi Arabia.
    Citation
    Hajjar, D. A. (2016). Bio-prospecting of Plants and Marine Organisms in Saudi Arabia for New Potential Bioactivity. KAUST Research Repository. https://doi.org/10.25781/KAUST-DQ642
    DOI
    10.25781/KAUST-DQ642
    ae974a485f413a2113503eed53cd6c53
    10.25781/KAUST-DQ642
    Scopus Count
    Collections
    PhD Dissertations; Physical Science and Engineering (PSE) Division; Chemical Engineering Program

    entitlement

     
    DSpace software copyright © 2002-2023  DuraSpace
    Quick Guide | Contact Us | KAUST University Library
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.