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dc.contributor.advisorChaieb, Saharoui
dc.contributor.authorNoutsi, Bakiza Kamal
dc.date.accessioned2016-12-06T13:30:47Z
dc.date.available2017-12-06T00:00:00Z
dc.date.issued2016-12
dc.identifier.citationNoutsi, B. K. (2016). Correlation between membrane fluidity cellular development and stem cell differentiation. KAUST Research Repository. https://doi.org/10.25781/KAUST-FX1J2
dc.identifier.doi10.25781/KAUST-FX1J2
dc.identifier.urihttp://hdl.handle.net/10754/621954
dc.description.abstractCell membranes are made up of a complex structure of lipids and proteins that diffuse laterally giving rise to what we call membrane fluidity. During cellular development, such as neuronal differentiation, cell membranes undergo dramatic structural changes induced by proteins such as ARC and Cofilin among others in the case of synaptic modification. In this study we used the generalized polarization (GP) property of fluorescent probe Laurdan using two-photon microscopy to determine membrane fluidity as a function of time and for various cell lines. A low GP value corresponds to a higher fluidity and a higher GP value is associated with a more rigid membrane. Four different cell lines were monitored such as hN2, NIH3T3, HEK293 and L6 cells. As expected, NIH3T3 cells have more rigid membrane at earlier stages of their development. On the other hand neurons tend to have the highest membrane fluidity early in their development emphasizing its correlation with plasticity and the need for this malleability during differentiation. This study sheds light on the involvement of membrane fluidity during neuronal differentiation and development of other cell lines.
dc.language.isoen
dc.subjectNeuron
dc.subjectdevelopment
dc.subjectstem cell
dc.subjectDifferentiation
dc.titleCorrelation between membrane fluidity cellular development and stem cell differentiation
dc.typeDissertation
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.rights.embargodate2017-12-06
thesis.degree.grantorKing Abdullah University of Science and Technology
dc.contributor.committeememberMagistretti, Pierre J.
dc.contributor.committeememberKhashab, Niveen M.
dc.contributor.committeememberGratton, Enrico
thesis.degree.disciplineBioscience
thesis.degree.nameDoctor of Philosophy
dc.rights.accessrightsAt the time of archiving, the student author of this dissertation opted to temporarily restrict access to it. The full text of this dissertation became available to the public after the expiration of the embargo on 2017-12-06.
refterms.dateFOA2017-12-06T00:00:00Z


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