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    Correlation between membrane fluidity cellular development and stem cell differentiation

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    Name:
    Pakiza Noutsi Dissertation.pdf
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    4.278Mb
    Format:
    PDF
    Description:
    Pakiza Noutsi Dissertation
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    Type
    Dissertation
    Authors
    Noutsi, Bakiza Kamal cc
    Advisors
    Chaieb, Saharoui cc
    Committee members
    Magistretti, Pierre J. cc
    Khashab, Niveen M. cc
    Gratton, Enrico
    Program
    Bioscience
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Date
    2016-12
    Embargo End Date
    2017-12-06
    Permanent link to this record
    http://hdl.handle.net/10754/621954
    
    Metadata
    Show full item record
    Access Restrictions
    At the time of archiving, the student author of this dissertation opted to temporarily restrict access to it. The full text of this dissertation became available to the public after the expiration of the embargo on 2017-12-06.
    Abstract
    Cell membranes are made up of a complex structure of lipids and proteins that diffuse laterally giving rise to what we call membrane fluidity. During cellular development, such as neuronal differentiation, cell membranes undergo dramatic structural changes induced by proteins such as ARC and Cofilin among others in the case of synaptic modification. In this study we used the generalized polarization (GP) property of fluorescent probe Laurdan using two-photon microscopy to determine membrane fluidity as a function of time and for various cell lines. A low GP value corresponds to a higher fluidity and a higher GP value is associated with a more rigid membrane. Four different cell lines were monitored such as hN2, NIH3T3, HEK293 and L6 cells. As expected, NIH3T3 cells have more rigid membrane at earlier stages of their development. On the other hand neurons tend to have the highest membrane fluidity early in their development emphasizing its correlation with plasticity and the need for this malleability during differentiation. This study sheds light on the involvement of membrane fluidity during neuronal differentiation and development of other cell lines.
    Citation
    Noutsi, B. K. (2016). Correlation between membrane fluidity cellular development and stem cell differentiation. KAUST Research Repository. https://doi.org/10.25781/KAUST-FX1J2
    DOI
    10.25781/KAUST-FX1J2
    ae974a485f413a2113503eed53cd6c53
    10.25781/KAUST-FX1J2
    Scopus Count
    Collections
    Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; PhD Dissertations

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