Correlation between membrane fluidity cellular development and stem cell differentiation
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Pakiza Noutsi Dissertation.pdf
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Pakiza Noutsi Dissertation
Type
DissertationAuthors
Noutsi, Bakiza Kamal
Advisors
Chaieb, Saharoui
Committee members
Magistretti, Pierre J.
Khashab, Niveen M.

Gratton, Enrico
Program
BioscienceKAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionDate
2016-12Embargo End Date
2017-12-06Permanent link to this record
http://hdl.handle.net/10754/621954
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At the time of archiving, the student author of this dissertation opted to temporarily restrict access to it. The full text of this dissertation became available to the public after the expiration of the embargo on 2017-12-06.Abstract
Cell membranes are made up of a complex structure of lipids and proteins that diffuse laterally giving rise to what we call membrane fluidity. During cellular development, such as neuronal differentiation, cell membranes undergo dramatic structural changes induced by proteins such as ARC and Cofilin among others in the case of synaptic modification. In this study we used the generalized polarization (GP) property of fluorescent probe Laurdan using two-photon microscopy to determine membrane fluidity as a function of time and for various cell lines. A low GP value corresponds to a higher fluidity and a higher GP value is associated with a more rigid membrane. Four different cell lines were monitored such as hN2, NIH3T3, HEK293 and L6 cells. As expected, NIH3T3 cells have more rigid membrane at earlier stages of their development. On the other hand neurons tend to have the highest membrane fluidity early in their development emphasizing its correlation with plasticity and the need for this malleability during differentiation. This study sheds light on the involvement of membrane fluidity during neuronal differentiation and development of other cell lines.Citation
Noutsi, B. K. (2016). Correlation between membrane fluidity cellular development and stem cell differentiation. KAUST Research Repository. https://doi.org/10.25781/KAUST-FX1J2ae974a485f413a2113503eed53cd6c53
10.25781/KAUST-FX1J2