On-Chip Magnetic Bead Manipulation and Detection Using a Magnetoresistive Sensor-Based Micro-Chip: Design Considerations and Experimental Characterization
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AbstractThe remarkable advantages micro-chip platforms offer over cumbersome, time-consuming equipment currently in use for bio-analysis are well documented. In this research, a micro-chip that includes a unique magnetic actuator (MA) for the manipulation of superparamagnetic beads (SPBs), and a magnetoresistive sensor for the detection of SPBs is presented. A design methodology, which takes into account the magnetic volume of SPBs, diffusion and heat transfer phenomena, is presented with the aid of numerical analysis to optimize the parameters of the MA. The MA was employed as a magnetic flux generator and experimental analysis with commercially available COMPEL™ and Dynabeads® demonstrated the ability of the MA to precisely transport a small number of SPBs over long distances and concentrate SPBs to a sensing site for detection. Moreover, the velocities of COMPEL™ and Dynabead® SPBs were correlated to their magnetic volumes and were in good agreement with numerical model predictions. We found that 2.8 μm Dynabeads® travel faster, and can be attracted to a magnetic source from a longer distance, than 6.2 μm COMPEL™ beads at magnetic flux magnitudes of less than 10 mT. The micro-chip system could easily be integrated with electronic circuitry and microfluidic functions, paving the way for an on-chip biomolecule quantification device
CitationGooneratne C, Kodzius R, Li F, Foulds I, Kosel J (2016) On-Chip Magnetic Bead Manipulation and Detection Using a Magnetoresistive Sensor-Based Micro-Chip: Design Considerations and Experimental Characterization. Sensors 16: 1369. Available: http://dx.doi.org/10.3390/s16091369.
SponsorsResearch reported in this publication was supported by King Abdullah University of Science and Technology (KAUST). The authors acknowledge Filipe Cardoso of INESC Microsistemas & Nanotecnologias (INESC MN) for his help with the TMR sensor fabrication. The authors would like to thank Ren Jian, Zhihong Wang, Basil Chew and Xiang Yu, for their help with microfabrication and material characterization. The authors would also like to thank Professor Nicole Pamme, Professor Quentin Pankhurst, Professor Martin Gijs, Professor Menno Prins, Mark Tarn and Chengxun Liu for fruitful discussions on the research
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