• Login
    View Item 
    •   Home
    • Research
    • Articles
    • View Item
    •   Home
    • Research
    • Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguideTheses and Dissertations LibguideSubmit an Item

    Statistics

    Display statistics

    Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Type
    Article
    Authors
    Dineshram, Ramadoss
    Chandramouli, Kondethimmanahalli cc
    Ko, Ginger Wai Kuen
    Zhang, Huoming cc
    Qian, Pei-Yuan cc
    Ravasi, Timothy cc
    Thiyagarajan, Vengatesen
    KAUST Department
    Applied Mathematics and Computational Science Program
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Computational Bioscience Research Center (CBRC)
    KAUST Environmental Epigenetics Research Program (KEEP)
    Proteomics and Protein Expression
    Date
    2016-03-17
    Online Publication Date
    2016-03-17
    Print Publication Date
    2016-06
    Permanent link to this record
    http://hdl.handle.net/10754/621623
    
    Metadata
    Show full item record
    Abstract
    The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression profiling using iTRAQ-LC-MS/MS identified more than 1300 proteins. Decreased pH had a negative effect on metamorphosis by down-regulating several proteins involved in energy production, metabolism, and protein synthesis. However, warming switched on these down-regulated pathways at pH 7.4. Under multiple stressors, cell signaling, energy production, growth, and developmental pathways were up-regulated, although metamorphosis was still reduced. Despite the lack of lethal effects, significant physiological responses to both individual and interacting climate change related stressors were observed at proteome level. The metamorphosing larvae of the C. gigas population in the Yellow Sea appear to have adequate phenotypic plasticity at the proteome level to survive in future coastal oceans, but with developmental and physiological costs. © 2016 John Wiley & Sons Ltd.
    Citation
    Dineshram R, Chandramouli K, Ko GWK, Zhang H, Qian P-Y, et al. (2016) Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology 22: 2054–2068. Available: http://dx.doi.org/10.1111/gcb.13249.
    Sponsors
    The authors would like to thank Ziniu Yu and Yang Zhang of South China Sea Institute of Oceanology for providing the oysters used in our experiments and for helping us with RT-PCR analysis. We thank Ivan Chiu (University of Hong Kong), and Yun Lam (City University of Hong Kong) for technical support and for providing access to sample preparation and data analysis. The collaboration between VT and PYQ is supported by the State Key Laboratory in Marine Pollution of City University of Hong Kong. We thank Gabriela Bindea of Cordeliers Research Center for adding Crassostrea gigas annotation resources in ClueGO for pathway analysis. We thank P. Sriyutha Murthy (BARC, Kalpakkam, India) for proofreading. We are also grateful to the three anonymous reviewers and the editor for their substantial contributions to this article. This study was funded by three GRF grants from the HKSAR-RGC (Grant Numbers: 705112P, 17304914, and 705511P).
    Publisher
    Wiley
    Journal
    Global Change Biology
    DOI
    10.1111/gcb.13249
    PubMed ID
    26990129
    Additional Links
    http://onlinelibrary.wiley.com/doi/10.1111/gcb.13249/full
    Relations
    Is Supplemented By:
    • [Dataset]
      Dineshram, R., Chandramouli, K., Ko, W. K. G., Zhang, H., Qian, P. Y., Ravasi, T., & Thiyagarajan, V. (2016). Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors, supplement to: Dineshram, R; Chandramouli, K; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen (2016): Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology, 22(6), 2054-2068 [Data set]. PANGAEA - Data Publisher for Earth & Environmental Science. https://doi.org/10.1594/pangaea.867318. DOI: 10.1594/PANGAEA.867318 HANDLE: 10754/624161
    ae974a485f413a2113503eed53cd6c53
    10.1111/gcb.13249
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Applied Mathematics and Computational Science Program; Computational Bioscience Research Center (CBRC)

    entitlement

    Related articles

    • Non-additive effects of ocean acidification in combination with warming on the larval proteome of the Pacific oyster, Crassostrea gigas.
    • Authors: Harney E, Artigaud S, Le Souchu P, Miner P, Corporeau C, Essid H, Pichereau V, Nunes FLD
    • Issue date: 2016 Mar 1
    • Interactive effects of ocean acidification, elevated temperature, and reduced salinity on early-life stages of the pacific oyster.
    • Authors: Ko GW, Dineshram R, Campanati C, Chan VB, Havenhand J, Thiyagarajan V
    • Issue date: 2014 Sep 2
    • Comparative and quantitative proteomics reveal the adaptive strategies of oyster larvae to ocean acidification.
    • Authors: Dineshram R, Quan Q, Sharma R, Chandramouli K, Yalamanchili HK, Chu I, Thiyagarajan V
    • Issue date: 2015 Dec
    • Proteomic response of marine invertebrate larvae to ocean acidification and hypoxia during metamorphosis and calcification.
    • Authors: Mukherjee J, Wong KK, Chandramouli KH, Qian PY, Leung PT, Wu RS, Thiyagarajan V
    • Issue date: 2013 Dec 15
    • Proteomic basis of stress responses in the gills of the pacific oyster Crassostrea gigas.
    • Authors: Zhang Y, Sun J, Mu H, Li J, Zhang Y, Xu F, Xiang Z, Qian PY, Qiu JW, Yu Z
    • Issue date: 2015 Jan 2
    DSpace software copyright © 2002-2022  DuraSpace
    Quick Guide | Contact Us | KAUST University Library
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.