Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors
Type
ArticleAuthors
Dineshram, RamadossChandramouli, Kondethimmanahalli

Ko, Ginger Wai Kuen
Zhang, Huoming

Qian, Pei-Yuan

Ravasi, Timothy

Thiyagarajan, Vengatesen
KAUST Department
Applied Mathematics and Computational Science ProgramBiological and Environmental Sciences and Engineering (BESE) Division
Bioscience Program
Computational Bioscience Research Center (CBRC)
KAUST Environmental Epigenetics Research Program (KEEP)
Proteomics and Protein Expression
Date
2016-03-17Online Publication Date
2016-03-17Print Publication Date
2016-06Permanent link to this record
http://hdl.handle.net/10754/621623
Metadata
Show full item recordAbstract
The metamorphosis of planktonic larvae of the Pacific oyster (Crassostrea gigas) underpins their complex life-history strategy by switching on the molecular machinery required for sessile life and building calcite shells. Metamorphosis becomes a survival bottleneck, which will be pressured by different anthropogenically induced climate change-related variables. Therefore, it is important to understand how metamorphosing larvae interact with emerging climate change stressors. To predict how larvae might be affected in a future ocean, we examined changes in the proteome of metamorphosing larvae under multiple stressors: decreased pH (pH 7.4), increased temperature (30 °C), and reduced salinity (15 psu). Quantitative protein expression profiling using iTRAQ-LC-MS/MS identified more than 1300 proteins. Decreased pH had a negative effect on metamorphosis by down-regulating several proteins involved in energy production, metabolism, and protein synthesis. However, warming switched on these down-regulated pathways at pH 7.4. Under multiple stressors, cell signaling, energy production, growth, and developmental pathways were up-regulated, although metamorphosis was still reduced. Despite the lack of lethal effects, significant physiological responses to both individual and interacting climate change related stressors were observed at proteome level. The metamorphosing larvae of the C. gigas population in the Yellow Sea appear to have adequate phenotypic plasticity at the proteome level to survive in future coastal oceans, but with developmental and physiological costs. © 2016 John Wiley & Sons Ltd.Citation
Dineshram R, Chandramouli K, Ko GWK, Zhang H, Qian P-Y, et al. (2016) Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology 22: 2054–2068. Available: http://dx.doi.org/10.1111/gcb.13249.Sponsors
The authors would like to thank Ziniu Yu and Yang Zhang of South China Sea Institute of Oceanology for providing the oysters used in our experiments and for helping us with RT-PCR analysis. We thank Ivan Chiu (University of Hong Kong), and Yun Lam (City University of Hong Kong) for technical support and for providing access to sample preparation and data analysis. The collaboration between VT and PYQ is supported by the State Key Laboratory in Marine Pollution of City University of Hong Kong. We thank Gabriela Bindea of Cordeliers Research Center for adding Crassostrea gigas annotation resources in ClueGO for pathway analysis. We thank P. Sriyutha Murthy (BARC, Kalpakkam, India) for proofreading. We are also grateful to the three anonymous reviewers and the editor for their substantial contributions to this article. This study was funded by three GRF grants from the HKSAR-RGC (Grant Numbers: 705112P, 17304914, and 705511P).Publisher
WileyJournal
Global Change BiologyPubMed ID
26990129Additional Links
http://onlinelibrary.wiley.com/doi/10.1111/gcb.13249/fullRelations
Is Supplemented By:- [Dataset]
Dineshram, R., Chandramouli, K., Ko, W. K. G., Zhang, H., Qian, P. Y., Ravasi, T., & Thiyagarajan, V. (2016). Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors, supplement to: Dineshram, R; Chandramouli, K; Ko, W K Ginger; Zhang, Huoming; Qian, Pei Yuan; Ravasi, Timothy; Thiyagarajan, Vengatesen (2016): Quantitative analysis of oyster larval proteome provides new insights into the effects of multiple climate change stressors. Global Change Biology, 22(6), 2054-2068 [Data set]. PANGAEA - Data Publisher for Earth & Environmental Science. https://doi.org/10.1594/pangaea.867318. DOI: 10.1594/PANGAEA.867318 HANDLE: 10754/624161
ae974a485f413a2113503eed53cd6c53
10.1111/gcb.13249
Scopus Count
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