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    Genetic improvement of tomato by targeted control of fruit softening

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    Gillis-GeneticImprovementTomato(AM).pdf
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    Description:
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    Type
    Article
    Authors
    Uluisik, Selman
    Chapman, Natalie H
    Smith, Rebecca
    Poole, Mervin
    Adams, Gary
    Gillis, Richard B
    Besong, Tabot M.D. cc
    Sheldon, Judith
    Stiegelmeyer, Suzy
    Perez, Laura
    Samsulrizal, Nurul
    Wang, Duoduo
    Fisk, Ian D cc
    Yang, Ni
    Baxter, Charles
    Rickett, Daniel
    Fray, Rupert
    Blanco-Ulate, Barbara
    Powell, Ann L T
    Harding, Stephen E
    Craigon, Jim
    Rose, Jocelyn K C
    Fich, Eric A
    Sun, Li
    Domozych, David S
    Fraser, Paul D
    Tucker, Gregory A
    Grierson, Don
    Seymour, Graham B cc
    KAUST Department
    KAUST Solar Center (KSC)
    Physical Science and Engineering (PSE) Division
    Date
    2016-07-25
    Online Publication Date
    2016-07-25
    Print Publication Date
    2016-09
    Permanent link to this record
    http://hdl.handle.net/10754/621550
    
    Metadata
    Show full item record
    Abstract
    Controlling the rate of softening to extend shelf life was a key target for researchers engineering genetically modified (GM) tomatoes in the 1990s, but only modest improvements were achieved. Hybrids grown nowadays contain 'non-ripening mutations' that slow ripening and improve shelf life, but adversely affect flavor and color. We report substantial, targeted control of tomato softening, without affecting other aspects of ripening, by silencing a gene encoding a pectate lyase. © 2016 Nature America, Inc. All rights reserved.
    Citation
    Uluisik S, Chapman NH, Smith R, Poole M, Adams G, et al. (2016) Genetic improvement of tomato by targeted control of fruit softening. Nature Biotechnology 34: 950–952. Available: http://dx.doi.org/10.1038/nbt.3602.
    Sponsors
    S.U. was funded by Ministry of Education of the Turkish Republic. The work was partly funded by BBSRC and Syngenta Seeds Ltd. through BBSRC 'stand-alone LINK' grants to P.D.F. and G.B.S. (BB/J015598/1 and BB/J016071/1). As part of the BBSRC grant, Syngenta staff (J.S., S.S., C.B. and D.R.) provided support with generating the transgenic plants, the bioinformatics analysis, the microscopy and writing the paper. G.B.S. and P.D.F. acknowledge support from EU project FP6 EUSOL and the European Cooperation in Science and Technology (COST) Action FA1106. D.D. was funded by US National Science Foundation grants NSF-MRI 1337280 and NSF-MRI 0922805. B.B.-U. and A.L.T.P. were funded by US National Science Foundation grants IOS 0957264 and IOS 0544504. J.R. was funded by a grant (IOS-1339287) from the Plant Genome Research Program of the US National Science Foundation. We acknowledge Syngenta Crop Protection, Research Triangle Park, North Carolina, USA, M. Franco for cDNA library preparation and J. Ni for RNASeq quality checks, read alignment and gene counting. We acknowledge J. Jones, V. Nekrasov and S. Kamoun, T.S.L. and The Gatsby Charitable Foundation for provision of the CRISPR-Cas 9 vectors. We also thank M. Bennett and J. Labavitch for useful discussions. COS<SUP>488</SUP> was kindly provided by J. Mravec and W.G.T. Willats of the Department of Plant and Environmental Sciences of the University of Copenhagen.
    Publisher
    Springer Science and Business Media LLC
    Journal
    Nature Biotechnology
    DOI
    10.1038/nbt.3602
    Additional Links
    https://shura.shu.ac.uk/31152/1/Gillis-GeneticImprovementTomato%28AM%29.pdf
    ae974a485f413a2113503eed53cd6c53
    10.1038/nbt.3602
    Scopus Count
    Collections
    Articles; Physical Science and Engineering (PSE) Division; KAUST Solar Center (KSC)

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