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dc.contributor.authorPiatek, Agnieszka Anna
dc.contributor.authorMahfouz, Magdy M.
dc.date.accessioned2016-11-03T08:30:09Z
dc.date.available2016-11-03T08:30:09Z
dc.date.issued2016-04-19
dc.identifier.citationPiatek A, Mahfouz MM (2016) Targeted genome regulation via synthetic programmable transcriptional regulators. Critical Reviews in Biotechnology: 1–12. Available: http://dx.doi.org/10.3109/07388551.2016.1165180.
dc.identifier.issn0738-8551
dc.identifier.issn1549-7801
dc.identifier.doi10.3109/07388551.2016.1165180
dc.identifier.urihttp://hdl.handle.net/10754/621471
dc.description.abstractRegulation of gene transcription controls cellular functions and coordinates responses to developmental, physiological and environmental cues. Precise and efficient molecular tools are needed to characterize the functions of single and multiple genes in linear and interacting pathways in a native context. Modular DNA-binding domains from zinc fingers (ZFs) and transcriptional activator-like proteins (TALE) are amenable to bioengineering to bind DNA target sequences of interest. As a result, ZF and TALE proteins were used to develop synthetic programmable transcription factors. However, these systems are limited by the requirement to re-engineer proteins for each new target sequence. The clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR associated 9 (Cas9) genome editing tool was recently repurposed for targeted transcriptional regulation by inactivation of the nuclease activity of Cas9. Due to the facile engineering, simplicity, precision and amenability to library construction, the CRISPR/Cas9 system is poised to revolutionize the functional genomics field across diverse eukaryotic species. In this review, we discuss the development of synthetic customizable transcriptional regulators and provide insights into their current and potential applications, with special emphasis on plant systems, in characterization of gene functions, elucidation of molecular mechanisms and their biotechnological applications. © 2016 Informa UK Limited, trading as Taylor & Francis Group
dc.description.sponsorshipKing Abdullah University of Science and Technology[CRG4]
dc.publisherInforma UK Limited
dc.subjectCRISPR/Cas9 system
dc.subjectfunctional genomics
dc.subjectsynthetic site-specific nucleases
dc.subjectsynthetic transcriptional regulators
dc.subjecttargeted genome regulation
dc.subjecttranscriptional activator-like effectors
dc.subjectzinc fingers
dc.titleTargeted genome regulation via synthetic programmable transcriptional regulators
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentPlant Science
dc.contributor.departmentPlant Science Program
dc.identifier.journalCritical Reviews in Biotechnology
kaust.personPiatek, Agnieszka Anna
kaust.personMahfouz, Magdy M.


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