Chloroplasts activity and PAP-signaling regulate programmed cell death in Arabidopsis
Chan, Kai Xun
Phua, Su Yin
Pogson, Barry J.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Plant Science Program
Desert Agriculture Initiative
MetadataShow full item record
AbstractProgrammed cell death (PCD) is a crucial process both for plant development and responses to biotic and abiotic stress. There is accumulating evidence that chloroplasts may play a central role during plant PCD as for mitochondria in animal cells, but it is still unclear whether they participate in PCD onset, execution, or both. To tackle this question, we have analyzed the contribution of chloroplast function to the cell death phenotype of the myoinositol phosphate synthase1 (mips1) mutant that forms spontaneous lesions in a light-dependent manner. We show that photosynthetically active chloroplasts are required for PCD to occur in mips1, but this process is independent of the redox state of the chloroplast. Systematic genetic analyses with retrograde signaling mutants reveal that 3’-phosphoadenosine 5’-phosphate, a chloroplast retrograde signal that modulates nuclear gene expression in response to stress, can inhibit cell death and compromises plant innate immunity via inhibition of the RNA-processing 5’-3’ exoribonucleases. Our results provide evidence for the role of chloroplast-derived signal and RNA metabolism in the control of cell death and biotic stress response. © 2016 American Society of Plant Biologists. All Rights Reserved.
CitationBruggeman Q, Mazubert C, Prunier F, Lugan R, Chan KX, et al. (2016) Chloroplasts activity and PAP-signaling regulate programmed cell death in Arabidopsis. Plant Physiol: pp.01872.2015. Available: http://dx.doi.org/10.1104/pp.15.01872.
SponsorsThis work was supported by the Agence Nationale de la Recherche (grant nos. ANR 2010 JCJC1207 01 and MAPK-IPS ANR-2010-BLAN-1613-02).
- Arabidopsis ACCELERATED CELL DEATH2 modulates programmed cell death.
- Authors: Yao N, Greenberg JT
- Issue date: 2006 Feb
- The role of autophagy in chloroplast degradation and chlorophagy in immune defenses during Pst DC3000 (AvrRps4) infection.
- Authors: Dong J, Chen W
- Issue date: 2013
- Evidence for a SAL1-PAP chloroplast retrograde pathway that functions in drought and high light signaling in Arabidopsis.
- Authors: Estavillo GM, Crisp PA, Pornsiriwong W, Wirtz M, Collinge D, Carrie C, Giraud E, Whelan J, David P, Javot H, Brearley C, Hell R, Marin E, Pogson BJ
- Issue date: 2011 Nov
- A pair of light signaling factors FHY3 and FAR1 regulates plant immunity by modulating chlorophyll biosynthesis.
- Authors: Wang W, Tang W, Ma T, Niu D, Jin JB, Wang H, Lin R
- Issue date: 2016 Jan
- The Arabidopsis thaliana Myo-inositol 1-phosphate synthase1 gene is required for Myo-inositol synthesis and suppression of cell death.
- Authors: Donahue JL, Alford SR, Torabinejad J, Kerwin RE, Nourbakhsh A, Ray WK, Hernick M, Huang X, Lyons BM, Hein PP, Gillaspy GE
- Issue date: 2010 Mar