The RNA Polymerase II C-Terminal Domain Phosphatase-Like Protein FIERY2/CPL1 Interacts with eIF4AIII and Is Essential for Nonsense-Mediated mRNA Decay in Arabidopsis

Cui, Peng; Chen, Tao; Qin, Tao; Ding, Feng; Wang, Zhenyu; Chen, Hao; Xiong, Liming

The RNA Polymerase II C-Terminal Domain Phosphatase-Like Protein FIERY2/CPL1 Interacts with eIF4AIII and Is Essential for Nonsense-Mediated mRNA Decay in Arabidopsis

Type

Article

Authors

Cui, Peng

Chen, Tao
Qin, Tao
Ding, Feng

Wang, Zhenyu
Chen, Hao
Xiong, Liming

KAUST Department

Biological and Environmental Science and Engineering (BESE) Division
Center for Desert Agriculture
Plant Science
Plant Stress Genomics Research Lab

KAUST Grant Number

BAS/1/1007-1-1
URF/1/2283-01-01

Online Publication Date

2016-02-17

Print Publication Date

2016-03

Date

2016-02-17

Abstract

© 2016 American Society of Plant Biologists. All rights reserved. Nonsense-mediated decay (NMD) is a posttranscriptional surveillance mechanism in eukaryotes that recognizes and degrades transcripts with premature translation-termination codons. The RNA polymerase II C-terminal domain phosphatase-like protein FIERY2 (FRY2; also known as C-TERMINAL DOMAIN PHOSPHATASE-LIKE1 [CPL1]) plays multiple roles in RNA processing in Arabidopsis thaliana. Here, we found that FRY2/CPL1 interacts with two NMD factors, eIF4AIII and UPF3, and is involved in the dephosphorylation of eIF4AIII. This dephosphorylation retains eIF4AIII in the nucleus and limits its accumulation in the cytoplasm. By analyzing RNA-seq data combined with quantitative RT-PCR validation, we found that a subset of alternatively spliced transcripts and 59-extended mRNAs with NMD-eliciting features accumulated in the fry2-1 mutant, cycloheximidetreated wild type, and upf3 mutant plants, indicating that FRY2 is essential for the degradation of these NMD transcripts.

Citation

Cui P, Chen T, Qin T, Ding F, Wang Z, et al. (2016) The RNA Polymerase II C-Terminal Domain Phosphatase-Like Protein FIERY2/CPL1 Interacts with eIF4AIII and Is Essential for Nonsense-Mediated mRNA Decay in Arabidopsis. Plant Cell 28: 770–785. Available: http://dx.doi.org/10.1105/tpc.15.00771.

Acknowledgements

This work was supported by King Abdullah University of Science and Technology Office of Sponsored Research (OSR) under Award URF/1/2283-01-01 and Faculty Baseline Funds BAS/1/1007-1-1. We thank the Bioscience Core Lab of KAUST for providing genome sequencing and imaging services.