The plant natriuretic peptide receptor is a guanylyl cyclase and enables cGMP-dependent signaling
MetadataShow full item record
AbstractThe functional homologues of vertebrate natriuretic peptides (NPs), the plant natriuretic peptides (PNPs), are a novel class of peptidic hormones that signal via guanosine 3′,5′-cyclic monophosphate (cGMP) and systemically affect plant salt and water balance and responses to biotrophic plant pathogens. Although there is increasing understanding of the complex roles of PNPs in plant responses at the systems level, little is known about the underlying signaling mechanisms. Here we report isolation and identification of a novel Leucine-Rich Repeat (LRR) protein that directly interacts with A. thaliana PNP, AtPNP-A. In vitro binding studies revealed that the Arabidopsis AtPNP-A binds specifically to the LRR protein, termed AtPNP-R1, and the active region of AtPNP-A is sufficient for the interaction to occur. Importantly, the cytosolic part of the AtPNP-R1, much like in some vertebrate NP receptors, harbors a catalytic center diagnostic for guanylyl cyclases and the recombinant AtPNP-R1 is capable of catalyzing the conversion of guanosine triphosphate to cGMP. In addition, we show that AtPNP-A causes rapid increases of cGMP levels in wild type (WT) leaf tissue while this response is significantly reduced in the atpnp-r1 mutants. AtPNP-A also causes cGMP-dependent net water uptake into WT protoplasts, and hence volume increases, whereas responses of the protoplasts from the receptor mutant are impaired. Taken together, our results suggest that the identified LRR protein is an AtPNP-A receptor essential for the PNP-dependent regulation of ion and water homeostasis in plants and that PNP- and vertebrate NP-receptors and their signaling mechanisms share surprising similarities. © 2016 Springer Science+Business Media Dordrecht
CitationTurek I, Gehring C (2016) The plant natriuretic peptide receptor is a guanylyl cyclase and enables cGMP-dependent signaling. Plant Molecular Biology 91: 275–286. Available: http://dx.doi.org/10.1007/s11103-016-0465-8.
SponsorsKing Abdullah University of Science and Technology
JournalPlant Molecular Biology
- Plant natriuretic peptides are apoplastic and paracrine stress response molecules.
- Authors: Wang YH, Gehring C, Irving HR
- Issue date: 2011 May
- Plant natriuretic peptides: systemic regulators of plant homeostasis and defense that can affect cardiomyoblasts.
- Authors: Gehring C, Irving H
- Issue date: 2013 Jun
- The Arabidopsis thaliana natriuretic peptide AtPNP-A is a systemic regulator of leaf dark respiration and signals via the phloem.
- Authors: Ruzvidzo O, Donaldson L, Valentine A, Gehring C
- Issue date: 2011 Sep 15
- The Arabidopsis thaliana proteome harbors undiscovered multi-domain molecules with functional guanylyl cyclase catalytic centers.
- Authors: Wong A, Gehring C
- Issue date: 2013 Jul 8
- Co-expression and promoter content analyses assign a role in biotic and abiotic stress responses to plant natriuretic peptides.
- Authors: Meier S, Bastian R, Donaldson L, Murray S, Bajic V, Gehring C
- Issue date: 2008 Feb 29