Compatibility analysis of 3D printer resin for biological applications
Khashab, Niveen M.
Salama, Khaled N.
KAUST DepartmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Biological and Environmental Sciences and Engineering (BESE) Division
Advanced Nanofabrication and Thin Film Core Lab
Physical Sciences and Engineering (PSE) Division
Chemical Science Program
Electrical Engineering Program
Permanent link to this recordhttp://hdl.handle.net/10754/621269
MetadataShow full item record
AbstractThe salient features of microfluidics such as reduced cost, handling small sample and reagent volumes and less time required to fabricate the devices has inspired the present work. The incompatibility of three-dimensional printer resins in their native form and the method to improve their compatibility to many biological processes via surface modification are reported. The compatibility of the material to build microfluidic devices was evaluated in three different ways: (i) determining if the ultraviolet (UV) cured resin inhibits the polymerase chain reaction (PCR), i.e. testing devices for PCR compatibility; (ii) observing agglutination complex formed on the surface of the UV cured resin when anti-C-reactive protein (CRP) antibodies and CRP proteins were allowed to agglutinate; and (iii) by culturing human embryonic kidney cell line cells and testing for its attachment and viability. It is shown that only a few among four in its native form could be used for fabrication of microchannels and that had the least effect on biological molecules that could be used for PCR and protein interactions and cells, whereas the others were used after treating the surface. Importance in building lab-on-chip/micrototal analysis systems and organ-on-chip devices is found.
CitationSivashankar S, Agambayev S, Alamoudi K, Buttner U, Khashab N, et al. (2016) Compatibility analysis of 3D printer resin for biological applications . Micro & Nano Letters 11: 654–659. Available: http://dx.doi.org/10.1049/mnl.2016.0530.
JournalMicro & Nano Letters
CollectionsArticles; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Imaging and Characterization Core Lab; Physical Sciences and Engineering (PSE) Division; Electrical Engineering Program; Chemical Science Program; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Except where otherwise noted, this item's license is described as This is an open access article published by the IET under the Creative Commons Attribution-NonCommercial-NoDerivs License
Showing items related by title, author, creator and subject.
Ultrafiltration Membrane Fouling and the Effect of Ion Exchange ResinsJamaly, Sanaa (2011-12) [Thesis]
Advisor: Croue, Jean-Philippe
Committee members: Amy, Gary L.; Zhang, TaoMembrane fouling is a challenging process for the ultrafiltration membrane during wastewater treatment. This research paper determines the organic character of foulants of different kinds of wastewater before and after adding some ion exchange resins. Two advanced organic characterization methods are compared in terms of concentration of dissolved organic carbons: The liquid chromatography with organic carbon (LC-OCD) and Shimadzu total organic carbon (TOC). In this study, two secondary wastewater effluents were treated using ultrafiltration membrane. To reduce fouling, pretreatment using some adsorbents were used in the study. Six ion exchange resins out of twenty were chosen to compare the effect of adsorbents on fouling membrane. Based on the percent of dissolved organic carbon’s removal, three adsorbents were determined to be the most efficient (DOWEX Marathon 11 anion exchange resin, DOWEX Optipore SD2 polymeric adsorbent, and DOWEX PSR2 anion exchange), and three other ones were determined to the least efficient (DOWEX Marathon A2 anion exchange resin, DOWEX SAR anion exchange resin, and DOWEX Optipore L493 polymeric adsorbent). Organic characterization for feed, permeate, and backwash samples were tested using LC-OCD and TOC to better understand the characteristics of foulants to prevent ultrafiltration membrane fouling. The results suggested that the polymeric ion exchange resin, DOWEX SD2, reduced fouling potential for both treated wastewaters. All the six ion exchange resins removed more humic fraction than other organic fractions in different percent, so this fraction is not the main for cause for UF membrane fouling. The fouling of colloids was tested before and after adding calcium. There is a severe fouling after adding Ca2+ to effluent colloids.
Characterization of solid UV curable 3D printer resins for biological applicationsSivashankar, Shilpa; Agambayev, Sumeyra; Buttner, Ulrich; Salama, Khaled N. (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS), Institute of Electrical and Electronics Engineers (IEEE), 2016-12-19) [Conference Paper]In this paper, we report a simple method to evaluate biocompatibility of solid UV cross-linked resin as a material for microfluidic devices that can be used for biological applications. We evaluated the biocompatibility of the material in two different ways (1) determining if the UV cured resin inhibits the polymerase chain reaction (PCR) and (2) observing agglutination complex formed on the surface of the UV cured resin when anti-CRP antibodies and C- reactive protein (CRP) proteins were allowed to agglutinate. Six different types of 3D printer resins were compared to test the biocompatibility. The study showed that only few among them could be used for fabrication of micro channels and that had least effect on biological molecules that could be used for PCR and protein interactions. Through these studies it is possible to estimate the curing time of various resin and their type of interaction with biomolecules. This study finds importance in on-chip tissue engineering and organ-on-chip applications.
Validation of High Pressure Resin Impregnation Technique for High Resolution Confocal Imaging of Geological SamplesHassan, Ahmed; Yutkin, M. P.; Chandra, Viswasanthi; Patzek, Tadeusz (SPE Middle East Oil and Gas Show and Conference, Society of Petroleum Engineers (SPE), 2019-03-13) [Conference Paper]In this paper, we present a procedure for high pressure resin impregnation of microporous rock. This procedure produces the high- quality pore casts that reveal the fine details of the complex pore space of micritic carbonates. We carefully test our resin impregnation procedure and demonstrate that it renders the high resolution, 3D confocal images of pore casts. In our work, we use silicon micromodels as a reference to validate the key parameters of high-pressure resin impregnation. We demonstrate possible artifacts and defects that might develop during rock impregnation with resin, e.g., the resin shrinkage and gas trapping. The main outcome of this paper is a robust protocol for obtaining the high-quality epoxy pore casts suitable for rock imaging with Confocal Laser Scanning Microscopy (CLSM). We have implemented this protocol and provided the high resolution, three-dimensional (3D) imagery and description of microporosity in micritic carbonates.