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dc.contributor.authorHabuchi, Satoshi
dc.contributor.authorYamamoto, Takuya
dc.contributor.authorTezuka, Yasuyuki
dc.date.accessioned2016-10-23T07:18:51Z
dc.date.available2016-10-23T07:18:51Z
dc.date.issued2016-09-26
dc.identifier.citationHabuchi S, Yamamoto T, Tezuka Y (2016) Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level. Journal of Visualized Experiments. Available: http://dx.doi.org/10.3791/54503.
dc.identifier.issn1940-087X
dc.identifier.doi10.3791/54503
dc.identifier.urihttp://hdl.handle.net/10754/621130
dc.description.abstractWe demonstrate a method for the synthesis of cyclic polymers and a protocol for characterizing their diffusive motion in a melt state at the single molecule level. An electrostatic self-assembly and covalent fixation (ESA-CF) process is used for the synthesis of the cyclic poly(tetrahydrofuran) (poly(THF)). The diffusive motion of individual cyclic polymer chains in a melt state is visualized using single molecule fluorescence imaging by incorporating a fluorophore unit in the cyclic chains. The diffusive motion of the chains is quantitatively characterized by means of a combination of mean-squared displacement (MSD) analysis and a cumulative distribution function (CDF) analysis. The cyclic polymer exhibits multiple-mode diffusion which is distinct from its linear counterpart. The results demonstrate that the diffusional heterogeneity of polymers that is often hidden behind ensemble averaging can be revealed by the efficient synthesis of the cyclic polymers using the ESA-CF process and the quantitative analysis of the diffusive motion at the single molecule level using the MSD and CDF analyses.
dc.description.sponsorshipThis work was supported by a Grant-in-Aid for Scientific Research No. 22750122 (S.H.), No. 26288099 (T.Y.), and No. 23350050 (Y.T.) of the Japan Society for the Promotion of Science. S.H. is grateful for The Kurata Memorial Hitachi Science and Technology Foundation. The research reported in this publication was supported by the King Abdullah University of Science and Technology (S.H.).
dc.publisherMyJove Corporation
dc.relation.urlhttp://www.jove.com/video/54503/synthesis-cyclic-polymers-characterization-their-diffusive-motion
dc.relation.urlhttp://www.jove.com/video/54503/
dc.rightsCopyright © 2016 Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License
dc.subjectChemistry
dc.subjectIssue 115
dc.subjectcyclic polymer
dc.subjectsynthesis
dc.subjectpolymer melt
dc.subjecttopology
dc.subjectdiffusion
dc.subjectsingle molecule
dc.subjectfluorescence microscopy
dc.titleSynthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.identifier.journalJournal of Visualized Experiments
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDivision of Applied Chemistry, Faculty of Engineering, Hokkaido University
dc.contributor.institutionDepartment of Organic and Polymeric Materials, Tokyo Institute of Technology
kaust.personHabuchi, Satoshi


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