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dc.contributor.authorAl Amoudi, Soha
dc.contributor.authorEssack, Magbubah
dc.contributor.authorSimoes, Marta
dc.contributor.authorBougouffa, Salim
dc.contributor.authorSoloviev, Irina
dc.contributor.authorArcher, John A.C.
dc.contributor.authorLafi, Feras Fawzi
dc.contributor.authorBajic, Vladimir B.
dc.date.accessioned2016-10-20T11:52:12Z
dc.date.available2016-10-20T11:52:12Z
dc.date.issued2016-09-10
dc.identifier.citationAl-Amoudi S, Essack M, Simões M, Bougouffa S, Soloviev I, et al. (2016) Bioprospecting Red Sea Coastal Ecosystems for Culturable Microorganisms and Their Antimicrobial Potential. Marine Drugs 14: 165. Available: http://dx.doi.org/10.3390/md14090165.
dc.identifier.issn1660-3397
dc.identifier.pmid27626430
dc.identifier.doi10.3390/md14090165
dc.identifier.urihttp://hdl.handle.net/10754/621094
dc.description.abstractMicroorganisms that inhabit unchartered unique soil such as in the highly saline and hot Red Sea lagoons on the Saudi Arabian coastline, represent untapped sources of potentially new bioactive compounds. In this study, a culture-dependent approach was applied to three types of sediments: mangrove mud (MN), microbial mat (MM), and barren soil (BS), collected from Rabigh harbor lagoon (RHL) and Al-Kharrar lagoon (AKL). The isolated bacteria were evaluated for their potential to produce bioactive compounds. The phylogenetic characterization of 251 bacterial isolates based on the 16S rRNA gene sequencing, supported their assignment to five different phyla: Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, and Planctomycetes. Fifteen putative novel species were identified based on a 16S rRNA gene sequence similarity to other strain sequences in the NCBI database, being ≤98%. We demonstrate that 49 of the 251 isolates exhibit the potential to produce antimicrobial compounds. Additionally, at least one type of biosynthetic gene sequence, responsible for the synthesis of secondary metabolites, was recovered from 25 of the 49 isolates. Moreover, 10 of the isolates had a growth inhibition effect towards Staphylococcus aureus, Salmonella typhimurium and Pseudomonas syringae. We report the previously unknown antimicrobial activity of B. borstelensis, P. dendritiformis and M. salipaludis against all three indicator pathogens. Our study demonstrates the evidence of diverse cultured microbes associated with the Red Sea harbor/lagoon environments and their potential to produce antimicrobial compounds.
dc.description.sponsorshipThe authors wish to acknowledge the experimental support from the King Abdullah University of Science and Technology (KAUST) Bioscience Core Laboratory. The computational analysis for this study was performed on Dragon and Snapdragon compute clusters of the Computational Bioscience Research Center at KAUST. The authors would also like to thank Christoph Gehring and his research group for their help and use of their instruments and equipment. We also thank Andre Antunes for his support and advice. This publication is based upon work supported by the KAUST Office of Sponsored Research (OSR) under Awards No URF/1/1976-02 and FCS/1/2448-01, and KAUST base research funds to V.B.B. Funding for open access charge: King Abdullah University of Science and Technology (CCF URF 1976).
dc.publisherMDPI AG
dc.relation.urlhttp://www.mdpi.com/1660-3397/14/9/165
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subjectRed Sea
dc.subjectmangrove mud
dc.subjectmicrobial mat
dc.subjectbarren soil
dc.subjectculturable bacteria
dc.subjectphylogenetic diversity
dc.subject16S rRNA
dc.subjectfirmicutes
dc.subjectbioactivity
dc.subjectantimicrobial compounds
dc.subjectbiosynthetic genes
dc.subjectbioinformatics
dc.titleBioprospecting Red Sea Coastal Ecosystems for Culturable Microorganisms and Their Antimicrobial Potential
dc.typeArticle
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentChemical and Biological Engineering Program
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentApplied Mathematics and Computational Science Program
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.identifier.journalMarine Drugs
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionBiology Department, Edge Hill University, St. Helens Road, Lancashire, Ormskirk L39 4QP, UK
kaust.personAl Amoudi, Soha
kaust.personEssack, Magbubah
kaust.personSimoes, Marta
kaust.personBougouffa, Salim
kaust.personSoloviev, Irina
kaust.personArcher, John A.C.
kaust.personLafi, Feras Fawzi
kaust.personBajic, Vladimir B.
kaust.grant.numberURF/1/1976-02
kaust.grant.numberFCS/1/2448-01
kaust.grant.numberCCF URF 1976
refterms.dateFOA2018-06-13T17:19:44Z


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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
Except where otherwise noted, this item's license is described as This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).