Bioprospecting Red Sea Coastal Ecosystems for Culturable Microorganisms and Their Antimicrobial Potential
AuthorsAl Amoudi, Soha
Archer, John A.C.
Lafi, Feras Fawzi
Bajic, Vladimir B.
KAUST DepartmentComputational Bioscience Research Center (CBRC)
Chemical and Biological Engineering Program
Biological and Environmental Sciences and Engineering (BESE) Division
Applied Mathematics and Computational Science Program
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/621094
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AbstractMicroorganisms that inhabit unchartered unique soil such as in the highly saline and hot Red Sea lagoons on the Saudi Arabian coastline, represent untapped sources of potentially new bioactive compounds. In this study, a culture-dependent approach was applied to three types of sediments: mangrove mud (MN), microbial mat (MM), and barren soil (BS), collected from Rabigh harbor lagoon (RHL) and Al-Kharrar lagoon (AKL). The isolated bacteria were evaluated for their potential to produce bioactive compounds. The phylogenetic characterization of 251 bacterial isolates based on the 16S rRNA gene sequencing, supported their assignment to five different phyla: Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, and Planctomycetes. Fifteen putative novel species were identified based on a 16S rRNA gene sequence similarity to other strain sequences in the NCBI database, being ≤98%. We demonstrate that 49 of the 251 isolates exhibit the potential to produce antimicrobial compounds. Additionally, at least one type of biosynthetic gene sequence, responsible for the synthesis of secondary metabolites, was recovered from 25 of the 49 isolates. Moreover, 10 of the isolates had a growth inhibition effect towards Staphylococcus aureus, Salmonella typhimurium and Pseudomonas syringae. We report the previously unknown antimicrobial activity of B. borstelensis, P. dendritiformis and M. salipaludis against all three indicator pathogens. Our study demonstrates the evidence of diverse cultured microbes associated with the Red Sea harbor/lagoon environments and their potential to produce antimicrobial compounds.
CitationAl-Amoudi S, Essack M, Simões M, Bougouffa S, Soloviev I, et al. (2016) Bioprospecting Red Sea Coastal Ecosystems for Culturable Microorganisms and Their Antimicrobial Potential. Marine Drugs 14: 165. Available: http://dx.doi.org/10.3390/md14090165.
SponsorsThe authors wish to acknowledge the experimental support from the King Abdullah University of Science and Technology (KAUST) Bioscience Core Laboratory. The computational analysis for this study was performed on Dragon and Snapdragon compute clusters of the Computational Bioscience Research Center at KAUST. The authors would also like to thank Christoph Gehring and his research group for their help and use of their instruments and equipment. We also thank Andre Antunes for his support and advice. This publication is based upon work supported by the KAUST Office of Sponsored Research (OSR) under Awards No URF/1/1976-02 and FCS/1/2448-01, and KAUST base research funds to V.B.B. Funding for open access charge: King Abdullah University of Science and Technology (CCF URF 1976).
CollectionsArticles; Biological and Environmental Sciences and Engineering (BESE) Division; Applied Mathematics and Computational Science Program; Chemical Engineering Program; Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Except where otherwise noted, this item's license is described as This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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