SLAH1, a homologue of the slow type anion channel SLAC1, modulates shoot Cl − accumulation and salt tolerance in Arabidopsis thaliana
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Desert Agriculture Initiative
Plant Science Program
Online Publication Date2016-06-23
Print Publication Date2016-08
Permanent link to this recordhttp://hdl.handle.net/10754/619766
MetadataShow full item record
AbstractSalinity tolerance is correlated with shoot chloride (Cl–) exclusion in multiple crops, but the molecular mechanisms of long-distance Cl– transport are poorly defined. Here, we characterize the in planta role of AtSLAH1 (a homologue of the slow type anion channel-associated 1 (SLAC1)). This protein, localized to the plasma membrane of root stelar cells, has its expression reduced by salt or ABA, which are key predictions for a protein involved with loading Cl– into the root xylem. Artificial microRNA knockdown mutants of AtSLAH1 had significantly reduced shoot Cl− accumulation when grown under low Cl–, whereas shoot Cl– increased and the shoot nitrate/chloride ratio decreased following AtSLAH1 constitutive or stelar-specific overexpression when grown in high Cl–. In both sets of overexpression lines a significant reduction in shoot biomass over the null segregants was observed under high Cl– supply, but not low Cl– supply. Further in planta data showed AtSLAH3 overexpression increased the shoot nitrate/chloride ratio, consistent with AtSLAH3 favouring nitrate transport. Heterologous expression of AtSLAH1 in Xenopus laevis oocytes led to no detectible transport, suggesting the need for post-translational modifications for AtSLAH1 to be active. Our in planta data are consistent with AtSLAH1 having a role in controlling root-to-shoot Cl– transport.
CitationSLAH1, a homologue of the slow type anion channel SLAC1, modulates shoot Cl − accumulation and salt tolerance in Arabidopsis thaliana 2016, 67 (15):4495 Journal of Experimental Botany
SponsorsThe authors thank Dr Darren Plett for donating the destination vector (pTOOL5) in this study; Yuan Li and Hui Zhou for performing the qRT-PCRs (Australian Centre for Plant Function Genomics, Adelaide, Australia). The work was supported by: the Grains Research and Development Corporation (UA000145 to S.J.R., M.G.); the Australian Research Council (ARC) through Centre of Excellence (CE14010008) and Future Fellowship (FT130100709) funding to M.G.; and, the University of Adelaide Graduate Research Scholarship and the Australian Centre for Plant Functional Genomics student scholarship to J.Q.
PublisherOxford University Press (OUP)
JournalJournal of Experimental Botany
- Silent S-Type Anion Channel Subunit SLAH1 Gates SLAH3 Open for Chloride Root-to-Shoot Translocation.
- Authors: Cubero-Font P, Maierhofer T, Jaslan J, Rosales MA, Espartero J, Díaz-Rueda P, Müller HM, Hürter AL, Al-Rasheid KA, Marten I, Hedrich R, Colmenero-Flores JM, Geiger D
- Issue date: 2016 Aug 22
- Identification of a Stelar-Localized Transport Protein That Facilitates Root-to-Shoot Transfer of Chloride in Arabidopsis.
- Authors: Li B, Byrt C, Qiu J, Baumann U, Hrmova M, Evrard A, Johnson AA, Birnbaum KD, Mayo GM, Jha D, Henderson SW, Tester M, Gilliham M, Roy SJ
- Issue date: 2016 Feb
- Shoot Na+ exclusion and increased salinity tolerance engineered by cell type-specific alteration of Na+ transport in Arabidopsis.
- Authors: Møller IS, Gilliham M, Jha D, Mayo GM, Roy SJ, Coates JC, Haseloff J, Tester M
- Issue date: 2009 Jul
- Nitrate-Dependent Control of Shoot K Homeostasis by the Nitrate Transporter1/Peptide Transporter Family Member NPF7.3/NRT1.5 and the Stelar K+ Outward Rectifier SKOR in Arabidopsis.
- Authors: Drechsler N, Zheng Y, Bohner A, Nobmann B, von Wirén N, Kunze R, Rausch C
- Issue date: 2015 Dec
- Grapevine and Arabidopsis Cation-Chloride Cotransporters Localize to the Golgi and Trans-Golgi Network and Indirectly Influence Long-Distance Ion Transport and Plant Salt Tolerance.
- Authors: Henderson SW, Wege S, Qiu J, Blackmore DH, Walker AR, Tyerman SD, Walker RR, Gilliham M
- Issue date: 2015 Nov