Genomic and Phenotypic Analyses Reveal the Emergence of an Atypical Salmonella enterica Serovar Senftenberg Variant in China
AuthorsAbd El Ghany, Moataz
Ansari, Hifzur Rahman
Hill-Cawthorne, Grant A.
Ho, Y. S.
Klena, John D.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Computational Bioscience Research Center (CBRC)
Biological and Environmental Sciences & Engineering Division (BESE)
Permanent link to this recordhttp://hdl.handle.net/10754/618379
MetadataShow full item record
AbstractHuman infections with Salmonella enterica subspecies enterica serovar Senftenberg are often associated with exposure to poultry flocks, farm environments, or contaminated food. The recent emergence of multidrug-resistant isolates has raised public health concerns. In this study, comparative genomics and phenotypic analysis were used to characterize 14 Salmonella Senftenberg clinical isolates recovered from multiple outbreaks in Shenzhen and Shanghai, China, between 2002 and 2011. Single-nucleotide polymorphism analyses identified two phylogenetically distinct clades of S. Senftenberg, designated SC1 and SC2, harboring variations in Salmonella pathogenicity island 1 (SPI-1) and SPI-2 and exhibiting distinct biochemical and phenotypic signatures. Although the two variants shared the same serotype, the SC2 isolates of sequence type 14 (ST14) harbored intact SPI-1 and -2 and hence were characterized by possessing efficient invasion capabilities. In contrast, the SC1 isolates had structural deletion patterns in both SPI-1 and -2 that correlated with an impaired capacity to invade cultured human cells and also the year of their isolation. These atypical SC1 isolates also lacked the capacity to produce hydrogen sulfide. These findings highlight the emergence of atypical Salmonella Senftenberg variants in China and provide genetic validation that variants lacking SPI-1 and regions of SPI-2, which leads to impaired invasion capacity, can still cause clinical disease. These data have identified an emerging public health concern and highlight the need to strengthen surveillance to detect the prevalence and transmission of nontyphoidal Salmonella species.
CitationGenomic and Phenotypic Analyses Reveal the Emergence of an Atypical Salmonella enterica Serovar Senftenberg Variant in China 2016, 54 (8):2014 Journal of Clinical Microbiology
SponsorsThis work, including the efforts of Qinghua Hu, was funded by The National Science Foundation of China (81071433). This work, including the efforts of Qinghua Hu, was funded by China National Science and Technology Major Project Foundation (2012ZX10004215-003-005 and 2016ZX10004215-005-005). This work, including the efforts of Qinghua Hu, was funded by Shenzhen Public Service Platform of Pathogenic Microorganisms Repository. This work, including the efforts of Arnab Pain, was funded by Faculty Baseline Research Funds (KAUST-BRF).
PublisherAmerican Society for Microbiology
JournalJournal of Clinical Microbiology
- Salmonella enterica serovar Senftenberg human clinical isolates lacking SPI-1.
- Authors: Hu Q, Coburn B, Deng W, Li Y, Shi X, Lan Q, Wang B, Coombes BK, Finlay BB
- Issue date: 2008 Apr
- Emergence and prevalence of non-H2S-producing Salmonella enterica serovar Senftenberg isolates belonging to novel sequence type 1751 in China.
- Authors: Yi S, Xie J, Liu N, Li P, Xu X, Li H, Sun J, Wang J, Liang B, Yang C, Wang X, Hao R, Wang L, Wu Z, Zhang J, Wang Y, Huang L, Sun Y, Klena JD, Meng J, Qiu S, Song H
- Issue date: 2014 Jul
- [Characteristics of Salmonella enterica serovar Senftenberg lacking Salmonella pathogenicity island 1].
- Authors: Li YH, Xu XB, Hu QH, Shi XL, Lin YM, Qiu YQ
- Issue date: 2011 Oct
- [Study on the epidemiological characteristics and molecular typing of Salmonella enterica subsp. enterica serovar Senftenberg in Shanghai].
- Authors: Xu XB, Yuan ZA, Jin HM, Xiao WJ, Gu BK, Chen M, Ran L, Diao BW, Cui ZG, Hu QH, Kan B
- Issue date: 2009 Sep
- Salmonella enterica serotype 4,5,12:i:-, an emerging Salmonella serotype that represents multiple distinct clones.
- Authors: Soyer Y, Moreno Switt A, Davis MA, Maurer J, McDonough PL, Schoonmaker-Bopp DJ, Dumas NB, Root T, Warnick LD, Gröhn YT, Wiedmann M
- Issue date: 2009 Nov