Magnetotactic Bacterial Cages as Safe and Smart Gene Delivery Vehicles
KAUST DepartmentSmart Hybrid Materials (SHMs) lab
Advanced Membranes and Porous Materials Center (AMPMC)
Computational Bioscience Research Center (CBRC)
Biological and Environmental Sciences and Engineering (BESE) Division
KAUST Advanced Nanofabrication Imaging and Characterization Core Laboratory
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AbstractIn spite of the huge advances in the area of synthetic carriers, their efficiency still poorly compares to natural vectors. Herein, we report the use of unmodified magnetotactic bacteria as a guidable delivery vehicle for DNA functionalized gold nanoparticles (AuNPs). High cargo loading is established under anaerobic conditions (bacteria is alive) through endocytosis where AuNPs are employed as transmembrane proteins mimics (facilitate endocytosis) as well as imaging agents to verify and quantify loading and release. The naturally bio-mineralized magnetosomes, within the bacteria, induce heat generation inside bacteria through magnetic hyperthermia. Most importantly after exposing the system to air (bacteria is dead) the cell wall stays intact providing an efficient bacterial vessel. Upon incubation with THP-1 cells, the magnetotactic bacterial cages (MBCs) adhere to the cell wall and are directly engulfed through the phagocytic activity of these cells. Applying magnetic hyperthermia leads to the dissociation of the bacterial microcarrier and eventual release of cargo.
CitationMagnetotactic Bacterial Cages as Safe and Smart Gene Delivery Vehicles 2016 OpenNano
SponsorsThe authors gratefully acknowledge King Abdullah University of Science and Technology (KAUST) for the support of this work. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.