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    Magnetotactic Bacterial Cages as Safe and Smart Gene Delivery Vehicles

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    Accepted Manuscript
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    Type
    Article
    Authors
    Alsaiari, Shahad K. cc
    Ezzeddine, Alaa
    Abdallah, Abdallah
    Sougrat, Rachid cc
    Khashab, Niveen M. cc
    KAUST Department
    Advanced Membranes and Porous Materials Research Center
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Chemical Science Program
    Computational Bioscience Research Center (CBRC)
    Electron Microscopy
    KAUST Advanced Nanofabrication Imaging and Characterization Core Laboratory
    Physical Science and Engineering (PSE) Division
    Sanger and Third Generation Sequencing
    Smart Hybrid Materials (SHMs) lab
    Date
    2016-07-28
    Online Publication Date
    2016-07-28
    Print Publication Date
    2016
    Permanent link to this record
    http://hdl.handle.net/10754/617788
    
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    Abstract
    In spite of the huge advances in the area of synthetic carriers, their efficiency still poorly compares to natural vectors. Herein, we report the use of unmodified magnetotactic bacteria as a guidable delivery vehicle for DNA functionalized gold nanoparticles (AuNPs). High cargo loading is established under anaerobic conditions (bacteria is alive) through endocytosis where AuNPs are employed as transmembrane proteins mimics (facilitate endocytosis) as well as imaging agents to verify and quantify loading and release. The naturally bio-mineralized magnetosomes, within the bacteria, induce heat generation inside bacteria through magnetic hyperthermia. Most importantly after exposing the system to air (bacteria is dead) the cell wall stays intact providing an efficient bacterial vessel. Upon incubation with THP-1 cells, the magnetotactic bacterial cages (MBCs) adhere to the cell wall and are directly engulfed through the phagocytic activity of these cells. Applying magnetic hyperthermia leads to the dissociation of the bacterial microcarrier and eventual release of cargo.
    Citation
    Magnetotactic Bacterial Cages as Safe and Smart Gene Delivery Vehicles 2016 OpenNano
    Sponsors
    The authors gratefully acknowledge King Abdullah University of Science and Technology (KAUST) for the support of this work. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
    Publisher
    Elsevier BV
    Journal
    OpenNano
    DOI
    10.1016/j.onano.2016.07.001
    Additional Links
    http://linkinghub.elsevier.com/retrieve/pii/S2352952016300093
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.onano.2016.07.001
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Advanced Membranes and Porous Materials Research Center; Physical Science and Engineering (PSE) Division; Chemical Science Program; Computational Bioscience Research Center (CBRC)

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