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dc.contributor.advisorPain, Arnab
dc.contributor.authorRchiad, ‍Zineb
dc.date.accessioned2016-07-26T11:51:34Z
dc.date.available2017-07-26T00:00:00Z
dc.date.issued2016-06
dc.identifier.citationRchiad, ‍Zineb. (2016). Transcriptome and microRNome of Theileria annulata Host Cells. KAUST Research Repository. https://doi.org/10.25781/KAUST-14ME0
dc.identifier.doi10.25781/KAUST-14ME0
dc.identifier.urihttp://hdl.handle.net/10754/617544
dc.description.abstractTropical Theileriosis is a parasitic disease of calves with a profound economic impact caused by Theileria annulata, an apicomplexan parasite of the genus Theileria. Transmitted by Hyalomma ticks, T. annulata infects and transforms bovine lymphocytes and macrophages into a cancer-like phenotype characterized by all six hallmarks of cancer. In the current study we investigate the transcriptional landscape of T. annulata-infected lymphocytes to define genes and miRNAs regulated by host cell transformation using next generation sequencing. We also define genes and miRNAs differentially expressed as a result of the attenuation of a T.annulata-infected macrophage cell line used as a vaccine. By comparing the transcriptional landscape of one attenuated and two transformed cell lines we identify four genes that we propose as key factors in transformation and virulence of the T. annulata host cells. We also identify miR- 126-5p as a key regulator of infected cells proliferation, adhesion, survival and invasiveness. In addition to the host cell trascriptome we studied T. annulata transcriptome and identified the role of ROS and TGF-β2 in controlling parasite gene expression. Moreover, we have used the deep parasite ssRNA-seq data to refine the available T. annulata annotation. Taken together, this study provides the full list of host cell’s genes and miRNAs transcriptionally perturbed after infection with T. annulata and after attenuation and describes genes and miRNAs never identified before as players in this type of host cell transformation. Moreover, this study provides the first database for the transcriptome of T. annulata and its host cells using next generation sequencing.
dc.language.isoen
dc.subjectTheileria
dc.subjectTranscriptome
dc.subjectmicroRNome
dc.subjectTransformation
dc.subjectattenuation
dc.titleTranscriptome and microRNome of Theileria annulata Host Cells
dc.typeDissertation
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.rights.embargodate2017-07-26
thesis.degree.grantorKing Abdullah University of Science and Technology
dc.contributor.committeememberMorrison, Ivan
dc.contributor.committeememberLangsley, Gordon
dc.contributor.committeememberMerzaban, Jasmeen
dc.contributor.committeememberGhaffour, NorEddine
thesis.degree.disciplineBioscience
thesis.degree.nameDoctor of Philosophy
dc.rights.accessrightsAt the time of archiving, the student author of this dissertation opted to temporarily restrict access to it. The full text of this dissertation became available to the public after the expiration of the embargo on 2017-07-26.
refterms.dateFOA2017-07-26T00:00:00Z


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