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    Transcriptome and microRNome of Theileria annulata Host Cells

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    Name:
    Zineb Rchiad Dissertation.pdf
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    102.1Mb
    Format:
    PDF
    Description:
    Zineb Rchiad Dissertation
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    Type
    Dissertation
    Authors
    Rchiad, ‍Zineb cc
    Advisors
    Pain, Arnab cc
    Committee members
    Morrison, Ivan
    Langsley, Gordon
    Merzaban, Jasmeen cc
    Ghaffour, NorEddine cc
    Program
    Bioscience
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Date
    2016-06
    Embargo End Date
    2017-07-26
    Permanent link to this record
    http://hdl.handle.net/10754/617544
    
    Metadata
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    Access Restrictions
    At the time of archiving, the student author of this dissertation opted to temporarily restrict access to it. The full text of this dissertation became available to the public after the expiration of the embargo on 2017-07-26.
    Abstract
    Tropical Theileriosis is a parasitic disease of calves with a profound economic impact caused by Theileria annulata, an apicomplexan parasite of the genus Theileria. Transmitted by Hyalomma ticks, T. annulata infects and transforms bovine lymphocytes and macrophages into a cancer-like phenotype characterized by all six hallmarks of cancer. In the current study we investigate the transcriptional landscape of T. annulata-infected lymphocytes to define genes and miRNAs regulated by host cell transformation using next generation sequencing. We also define genes and miRNAs differentially expressed as a result of the attenuation of a T.annulata-infected macrophage cell line used as a vaccine. By comparing the transcriptional landscape of one attenuated and two transformed cell lines we identify four genes that we propose as key factors in transformation and virulence of the T. annulata host cells. We also identify miR- 126-5p as a key regulator of infected cells proliferation, adhesion, survival and invasiveness. In addition to the host cell trascriptome we studied T. annulata transcriptome and identified the role of ROS and TGF-β2 in controlling parasite gene expression. Moreover, we have used the deep parasite ssRNA-seq data to refine the available T. annulata annotation. Taken together, this study provides the full list of host cell’s genes and miRNAs transcriptionally perturbed after infection with T. annulata and after attenuation and describes genes and miRNAs never identified before as players in this type of host cell transformation. Moreover, this study provides the first database for the transcriptome of T. annulata and its host cells using next generation sequencing.
    Citation
    Rchiad, ‍Zineb. (2016). Transcriptome and microRNome of Theileria annulata Host Cells. KAUST Research Repository. https://doi.org/10.25781/KAUST-14ME0
    DOI
    10.25781/KAUST-14ME0
    ae974a485f413a2113503eed53cd6c53
    10.25781/KAUST-14ME0
    Scopus Count
    Collections
    Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; PhD Dissertations

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