A novel role for pigment genes in the stress response in rainbow trout (Oncorhynchus mykiss)
AuthorsKhan, Uniza Wahid
Hinkle, Patricia M.
Pasha, Farhan Ahmad
Johansen, Ida Beitnes
Silva, Patricia I. M.
Omholt, Stig W.
Våge, Dag Inge
KAUST DepartmentKAUST Catalysis Center (KCC)
Online Publication Date2016-07-04
Print Publication Date2016-09
Permanent link to this recordhttp://hdl.handle.net/10754/615930
MetadataShow full item record
AbstractIn many vertebrate species visible melanin-based pigmentation patterns correlate with high stress- and disease-resistance, but proximate mechanisms for this trait association remain enigmatic. Here we show that a missense mutation in a classical pigmentation gene, melanocyte stimulating hormone receptor (MC1R), is strongly associated with distinct differences in steroidogenic melanocortin 2 receptor (MC2R) mRNA expression between high- (HR) and low-responsive (LR) rainbow trout (Oncorhynchus mykiss). We also show experimentally that cortisol implants increase the expression of agouti signaling protein (ASIP) mRNA in skin, likely explaining the association between HR-traits and reduced skin melanin patterning. Molecular dynamics simulations predict that melanocortin 2 receptor accessory protein (MRAP), needed for MC2R function, binds differently to the two MC1R variants. Considering that mRNA for MC2R and the MC1R variants are present in head kidney cells, we hypothesized that MC2R activity is modulated in part by different binding affinities of the MC1R variants for MRAP. Experiments in mammalian cells confirmed that trout MRAP interacts with the two trout MC1R variants and MC2R, but failed to detect regulation of MC2R signaling, possibly due to high constitutive MC1R activity.
CitationA novel role for pigment genes in the stress response in rainbow trout (Oncorhynchus mykiss) 2016, 6:28969 Scientific Reports
SponsorsWe are deeply thankful to Tom Pottinger for making the HR-LR rainbow trout strains available for this study. We thank Jon K. Lærdahl for making a structural model of MC1R, Christina Sørensen for helping with the cortisol analysis and Ida G. Lunde for help with the figures. Primer and competitor design for the real competitive PCR analysis was kindly performed by Sequenom Custom Service, Hamburg, Germany. The project was financially supported by the Norwegian University of Life Sciences, by Research Council of Norway projects no. 172609, 199728, 218534 and by National Institutes of Health Grant DK19974 (P.M.H.). Atlantic Salmon genomic sequences used for primer design was kindly provided by the International Cooperation to Sequence the Atlantic Salmon Genome (ICSASG).
- Cadmium disrupts melanocortin 2 receptor signaling in rainbow trout.
- Authors: Sandhu N, Liang L, McGeer J, Dores RM, Vijayan MM
- Issue date: 2019 Apr
- Molecular characterization, tissue-specific expression, and regulation of melanocortin 2 receptor in rainbow trout.
- Authors: Aluru N, Vijayan MM
- Issue date: 2008 Sep
- Cadmium-mediated disruption of cortisol biosynthesis involves suppression of corticosteroidogenic genes in rainbow trout.
- Authors: Sandhu N, Vijayan MM
- Issue date: 2011 May
- Identifying the activation motif in the N-terminal of rainbow trout and zebrafish melanocortin-2 receptor accessory protein 1 (MRAP1) orthologs.
- Authors: Dores RM, Liang L, Hollmann RE, Sandhu N, Vijayan MM
- Issue date: 2016 Aug 1
- Functional expression of frog and rainbow trout melanocortin 2 receptors using heterologous MRAP1s.
- Authors: Liang L, Sebag JA, Eagelston L, Serasinghe MN, Veo K, Reinick C, Angleson J, Hinkle PM, Dores RM
- Issue date: 2011 Oct 1