Show simple item record

dc.contributor.authorCima, Igor
dc.contributor.authorKong, Say Li
dc.contributor.authorSengupta, Debarka
dc.contributor.authorTan, Iain B
dc.contributor.authorPhyo, Wai Min
dc.contributor.authorLee, Daniel
dc.contributor.authorHu, Min
dc.contributor.authorIliescu, Ciprian
dc.contributor.authorAlexander, Irina
dc.contributor.authorGoh, Wei Lin
dc.contributor.authorRahmani, Mehran
dc.contributor.authorSuhaimi, Nur-Afidah Mohamed
dc.contributor.authorVo, Jess H
dc.contributor.authorTai, Joyce A
dc.contributor.authorTan, Joanna H
dc.contributor.authorChua, Clarinda
dc.contributor.authorTen, Rachel
dc.contributor.authorLim, Wan Jun
dc.contributor.authorChew, Min Hoe
dc.contributor.authorHauser, Charlotte
dc.contributor.authorvan Dam, Rob M
dc.contributor.authorLim, Wei-Yen
dc.contributor.authorPrabhakar, Shyam
dc.contributor.authorLim, Bing
dc.contributor.authorKoh, Poh Koon
dc.contributor.authorRobson, Paul
dc.contributor.authorYing, Jackie Y
dc.contributor.authorHillmer, Axel M
dc.contributor.authorTan, Min-Han
dc.date.accessioned2016-07-10T08:04:32Z
dc.date.available2016-07-10T08:04:32Z
dc.date.issued2016-06-29
dc.identifier.citationTumor-derived circulating endothelial cell clusters in colorectal cancer. 2016, 8 (345):345ra89 Sci Transl Med
dc.identifier.issn1946-6242
dc.identifier.pmid27358499
dc.identifier.doi10.1126/scitranslmed.aad7369
dc.identifier.urihttp://hdl.handle.net/10754/615874
dc.description.abstractClusters of tumor cells are often observed in the blood of cancer patients. These structures have been described as malignant entities for more than 50 years, although their comprehensive characterization is lacking. Contrary to current consensus, we demonstrate that a discrete population of circulating cell clusters isolated from the blood of colorectal cancer patients are not cancerous but consist of tumor-derived endothelial cells. These clusters express both epithelial and mesenchymal markers, consistent with previous reports on circulating tumor cell (CTC) phenotyping. However, unlike CTCs, they do not mirror the genetic variations of matched tumors. Transcriptomic analysis of single clusters revealed that these structures exhibit an endothelial phenotype and can be traced back to the tumor endothelium. Further results show that tumor-derived endothelial clusters do not form by coagulation or by outgrowth of single circulating endothelial cells, supporting a direct release of clusters from the tumor vasculature. The isolation and enumeration of these benign clusters distinguished healthy volunteers from treatment-naïve as well as pathological early-stage (≤IIA) colorectal cancer patients with high accuracy, suggesting that tumor-derived circulating endothelial cell clusters could be used as a means of noninvasive screening for colorectal cancer. In contrast to CTCs, tumor-derived endothelial cell clusters may also provide important information about the underlying tumor vasculature at the time of diagnosis, during treatment, and throughout the course of the disease.
dc.language.isoen
dc.language.isoen
dc.publisherAmerican Association for the Advancement of Science (AAAS)
dc.rightsArchived with thanks to Science translational medicine
dc.subjectColorectal cancer
dc.subjectEndothelial cells
dc.subjectTumor cells
dc.subjectBiomarker
dc.subjectCirculating Tumor Cells
dc.subjectSingle cell analysis
dc.titleTumor-derived circulating endothelial cell clusters in colorectal cancer.
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.identifier.journalScience Translational Medicine
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionInstitute of Bioengineering and Nanotechnology, Singapore 138669, Singapore
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personCima, Igor
kaust.personAlexander, Irina
kaust.personHauser, Charlotte
refterms.dateFOA2016-12-29T00:00:00Z
dc.date.published-online2016-06-29
dc.date.published-print2016-06-29


Files in this item

Thumbnail
Name:
345ra89.full.pdf
Size:
1.249Mb
Format:
PDF
Description:
Article
Thumbnail
Name:
Cima_aad7369_SM.PDF
Size:
4.027Mb
Format:
PDF
Description:
Supplementary Materials
Thumbnail
Name:
aad7369_SupplementalTables_S1-S14 (1).xls
Size:
14.07Mb
Format:
Microsoft Excel
Description:
Supplementary Tables

This item appears in the following Collection(s)

Show simple item record