Tumor-derived circulating endothelial cell clusters in colorectal cancer.
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Type
ArticleAuthors
Cima, IgorKong, Say Li
Sengupta, Debarka
Tan, Iain B
Phyo, Wai Min
Lee, Daniel
Hu, Min
Iliescu, Ciprian
Alexander, Irina
Goh, Wei Lin
Rahmani, Mehran
Suhaimi, Nur-Afidah Mohamed
Vo, Jess H
Tai, Joyce A
Tan, Joanna H
Chua, Clarinda
Ten, Rachel
Lim, Wan Jun
Chew, Min Hoe
Hauser, Charlotte

van Dam, Rob M
Lim, Wei-Yen
Prabhakar, Shyam
Lim, Bing
Koh, Poh Koon
Robson, Paul
Ying, Jackie Y
Hillmer, Axel M
Tan, Min-Han
KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Computational Bioscience Research Center (CBRC)
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Date
2016-06-29Online Publication Date
2016-06-29Print Publication Date
2016-06-29Permanent link to this record
http://hdl.handle.net/10754/615874
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Show full item recordAbstract
Clusters of tumor cells are often observed in the blood of cancer patients. These structures have been described as malignant entities for more than 50 years, although their comprehensive characterization is lacking. Contrary to current consensus, we demonstrate that a discrete population of circulating cell clusters isolated from the blood of colorectal cancer patients are not cancerous but consist of tumor-derived endothelial cells. These clusters express both epithelial and mesenchymal markers, consistent with previous reports on circulating tumor cell (CTC) phenotyping. However, unlike CTCs, they do not mirror the genetic variations of matched tumors. Transcriptomic analysis of single clusters revealed that these structures exhibit an endothelial phenotype and can be traced back to the tumor endothelium. Further results show that tumor-derived endothelial clusters do not form by coagulation or by outgrowth of single circulating endothelial cells, supporting a direct release of clusters from the tumor vasculature. The isolation and enumeration of these benign clusters distinguished healthy volunteers from treatment-naïve as well as pathological early-stage (≤IIA) colorectal cancer patients with high accuracy, suggesting that tumor-derived circulating endothelial cell clusters could be used as a means of noninvasive screening for colorectal cancer. In contrast to CTCs, tumor-derived endothelial cell clusters may also provide important information about the underlying tumor vasculature at the time of diagnosis, during treatment, and throughout the course of the disease.Citation
Tumor-derived circulating endothelial cell clusters in colorectal cancer. 2016, 8 (345):345ra89 Sci Transl MedJournal
Science Translational MedicinePubMed ID
27358499ae974a485f413a2113503eed53cd6c53
10.1126/scitranslmed.aad7369
Scopus Count
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