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dc.contributor.authorBagchi, Samik
dc.contributor.authorLamendella, Regina
dc.contributor.authorStrutt, Steven
dc.contributor.authorvan Loosdrecht, Mark C.M.
dc.contributor.authorSaikaly, Pascal
dc.date.accessioned2016-06-23T10:40:40Z
dc.date.available2016-06-23T10:40:40Z
dc.date.issued2016-06-20
dc.identifier.citationMetatranscriptomics reveals the molecular mechanism of large granule formation in granular anammox reactor 2016, 6:28327 Scientific Reports
dc.identifier.issn2045-2322
dc.identifier.pmid27319320
dc.identifier.doi10.1038/srep28327
dc.identifier.urihttp://hdl.handle.net/10754/614405
dc.description.abstractGranules enriched with anammox bacteria are essential in enhancing the treatment of ammonia-rich wastewater, but little is known about how anammox bacteria grow and multiply inside granules. Here, we combined metatranscriptomics, quantitative PCR and 16S rRNA gene sequencing to study the changes in community composition, metabolic gene content and gene expression in a granular anammox reactor with the objective of understanding the molecular mechanism of anammox growth and multiplication that led to formation of large granules. Size distribution analysis revealed the spatial distribution of granules in which large granules having higher abundance of anammox bacteria (genus Brocadia) dominated the bottom biomass. Metatranscriptomics analysis detected all the essential transcripts for anammox metabolism. During the later stage of reactor operation, higher expression of ammonia and nitrite transport proteins and key metabolic enzymes mainly in the bottom large granules facilitated anammox bacteria activity. The high activity resulted in higher growth and multiplication of anammox bacteria and expanded the size of the granules. This conceptual model for large granule formation proposed here may assist in the future design of anammox processes for mainstream wastewater treatment.
dc.description.sponsorshipThis research was funded by Competitive Research Grant (CRG_R2_13_SAIK_KAUST_1) from King Abdullah University of Science and Technology (KAUST). Special thanks are extended to Shan Sun for assisting with the qPCR analysis and Academic Writing Service Team at KAUST for making illustration figure and animation. We also thank Shahjahan Ali and colleagues in the Bioscience Core Laboratory at KAUST for their support in metatranscriptome sequencing on the Illumina HiSeq and in 16S rRNA gene sequencing on the Ion Torrent PGM. A portion of the bioinformatics analyses was supported by a grant to Juniata College from the Howard Hughes Medical Institute (http://www.hhmi.org) through the Precollege and Undergraduate Science Education Program.
dc.language.isoen
dc.publisherSpringer Nature
dc.relation.urlhttp://www.nature.com/articles/srep28327
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
dc.titleMetatranscriptomics reveals the molecular mechanism of large granule formation in granular anammox reactor
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentEnvironmental Science and Engineering Program
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)
dc.identifier.journalScientific Reports
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionJuniata College, Biology Department, Huntingdon, PA 16652, USA
dc.contributor.institutionDelft University of Technology, Environmental Biotechnology, Department of Biotechnology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands
dc.contributor.institutionUniversity of Kansas, Department of Civil, Environmental, and Architectural Engineering, Lawrence, KS, USA 66045.
dc.contributor.institutionUniversity of California, Berkeley, Department of Molecular and Cell Biology, Berkeley, CA 94720, USA.
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personBagchi, Samik
kaust.personSaikaly, Pascal
kaust.grant.numberCRG_R2_13_SAIK_KAUST_1
refterms.dateFOA2018-06-14T05:56:11Z
kaust.acknowledged.supportUnitBioscience Core Laboratory
kaust.acknowledged.supportUnitCompetitive Research
dc.date.published-online2016-06-20
dc.date.published-print2016-09


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