Translational repression of the cpw-wpc gene family in the malaria parasite Plasmodium
KAUST DepartmentPathogen Genomics Laboratory
Biological and Environmental Sciences and Engineering (BESE) Division
MetadataShow full item record
AbstractThe technical challenges of working with the sexual stages of the malaria parasite Plasmodium have hindered the characterization of sexual stage antigens in the quest for a successful malaria transmission-blocking vaccine. One such predicted and largely uncharacterized group of sexual stage candidate antigens is the CPW-WPC family of proteins. CPW-WPC proteins are named for a characteristic domain that contains two conserved motifs, CPxxW and WPC. Conserved across Apicomplexa, this family is also present earlier in the Alveolata in the free-living, non-parasitophorous, photosynthetic chromerids, Chromera and Vitrella. In P. falciparum and P. berghei blood stage parasites the transcripts of all nine cpw-wpc genes have been detected in gametocytes. RNA immunoprecipitation followed by reverse transcriptase-PCR reveals all P. berghei cpw-wpc transcripts to be bound by the translational repressors DOZI and CITH, and thus are likely under translational control prior to transmission from the rodent host to the mosquito vector in P. berghei. The GFP tagging of two endogenous P. berghei genes confirmed translational silencing in the gametocyte and translation in ookinetes. Establishing a luciferase transgene assay we show that the 3′ untranslated region of PF3D7_1331400 controls protein expression of this reporter in P. falciparum gametocytes. Our analyses suggest that cpw-wpc genes are translationally silenced in gametocytes across Plasmodium spp. and activated during ookinete formation and thus may have a role in transmission to the mosquito.
CitationTranslational repression of the cpw-wpc gene family in the malaria parasite Plasmodium 2016 Parasitology International
SponsorsThe authors would like to thank Dr. Friedrich Frischknecht, Dr. Photini Sinnis, Dr. Alida Coppi, Dr. Kirk Deitsch, Dr. Cristina Bancells and Dr. Cristina Moreira for their suggestions and help with experiments. This work received financial support from Fundação para a Ciência e a Tecnologia (FCT) of the Portuguese Ministry of Science, Technology and High Education and the British Council; Jorge M. Santos was supported by an FCT Ph.D. Fellowship (SFRH/BD/63849/2009). Dr. Gunnar R. Mair was supported by FCT projectsPTDC/BIA-BCM/105610/2008 and PTDC/SAU-MIC/122082/2010. Dr. Thomas Templeton was supported by the NIH/NIAID grant 1R01AI080754-01 A1, the William Randolph Hearst Foundation, and a visiting professorship to the Institute of Tropical Medicine, Nagasaki University, Japan.
- A member of the CPW-WPC protein family is expressed in and localized to the surface of developing ookinetes.
- Authors: Kangwanrangsan N, Tachibana M, Jenwithisuk R, Tsuboi T, Riengrojpitak S, Torii M, Ishino T
- Issue date: 2013 Apr 15
- Genome-wide RIP-Chip analysis of translational repressor-bound mRNAs in the Plasmodium gametocyte.
- Authors: Guerreiro A, Deligianni E, Santos JM, Silva PA, Louis C, Pain A, Janse CJ, Franke-Fayard B, Carret CK, Siden-Kiamos I, Mair GR
- Issue date: 2014 Nov 3
- Characterization of a Plasmodium berghei sexual stage antigen PbPH as a new candidate for malaria transmission-blocking vaccine.
- Authors: Kou X, Zheng W, Du F, Liu F, Wang M, Fan Q, Cui L, Luo E, Cao Y
- Issue date: 2016 Apr 2
- Changes in the transcriptome of the malaria parasite Plasmodium falciparum during the initial phase of transmission from the human to the mosquito.
- Authors: Ngwa CJ, Scheuermayer M, Mair GR, Kern S, Brügl T, Wirth CC, Aminake MN, Wiesner J, Fischer R, Vilcinskas A, Pradel G
- Issue date: 2013 Apr 15
- Transgenic expression of a mosquito-stage malarial protein, Pbs21, in blood stages of transformed Plasmodium berghei and induction of an immune response upon infection.
- Authors: Margos G, van Dijk MR, Ramesar J, Janse CJ, Waters AP, Sinden RE
- Issue date: 1998 Aug