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dc.contributor.authorMichoud, Gregoire
dc.contributor.authorJebbar, Mohamed
dc.date.accessioned2016-06-06T07:30:13Z
dc.date.available2016-06-06T07:30:13Z
dc.date.issued2016-06-02
dc.identifier.citationHigh hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii 2016, 6:27289 Scientific Reports
dc.identifier.issn2045-2322
dc.identifier.doi10.1038/srep27289
dc.identifier.urihttp://hdl.handle.net/10754/611779
dc.description.abstractPyrococcus yayanosii CH1, as the first and only obligate piezophilic hyperthermophilic microorganism discovered to date, extends the physical and chemical limits of life on Earth. It was isolated from the Ashadze hydrothermal vent at 4,100 m depth. Multi-omics analyses were performed to study the mechanisms used by the cell to cope with high hydrostatic pressure variations. In silico analyses showed that the P. yayanosii genome is highly adapted to its harsh environment, with a loss of aromatic amino acid biosynthesis pathways and the high constitutive expression of the energy metabolism compared with other non-obligate piezophilic Pyrococcus species. Differential proteomics and transcriptomics analyses identified key hydrostatic pressure-responsive genes involved in translation, chemotaxis, energy metabolism (hydrogenases and formate metabolism) and Clustered Regularly Interspaced Short Palindromic Repeats sequences associated with Cellular apoptosis susceptibility proteins.
dc.description.sponsorshipThe authors would like to thank Didier Chevret for performing the proteomic analysis and Mickael Beauverger for help with protein extraction and gel migration. We are indebted to Helen McCombie for her helpful language improvement. This work was supported by the Agence Nationale de la Recherche (ANR-10-BLAN-1725 01-Living deep) and G.M. was supported by a PhD fellowship from the Conseil Régional de Bretagne.
dc.language.isoen
dc.publisherSpringer Nature
dc.relation.urlhttp://www.nature.com/articles/srep27289
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
dc.titleHigh hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalScientific Reports
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionUniv Brest, CNRS, Ifremer, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Institut Universitaire Européen de la Mer (IUEM), rue Dumont d’Urville, 29 280 Plouzané, France
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personMichoud, Gregoire
refterms.dateFOA2018-06-13T11:55:46Z
dc.date.published-online2016-06-02
dc.date.published-print2016-07


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