High hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii
Type
ArticleAuthors
Michoud, Gregoire
Jebbar, Mohamed

Date
2016-06-02Online Publication Date
2016-06-02Print Publication Date
2016-07Permanent link to this record
http://hdl.handle.net/10754/611779
Metadata
Show full item recordAbstract
Pyrococcus yayanosii CH1, as the first and only obligate piezophilic hyperthermophilic microorganism discovered to date, extends the physical and chemical limits of life on Earth. It was isolated from the Ashadze hydrothermal vent at 4,100 m depth. Multi-omics analyses were performed to study the mechanisms used by the cell to cope with high hydrostatic pressure variations. In silico analyses showed that the P. yayanosii genome is highly adapted to its harsh environment, with a loss of aromatic amino acid biosynthesis pathways and the high constitutive expression of the energy metabolism compared with other non-obligate piezophilic Pyrococcus species. Differential proteomics and transcriptomics analyses identified key hydrostatic pressure-responsive genes involved in translation, chemotaxis, energy metabolism (hydrogenases and formate metabolism) and Clustered Regularly Interspaced Short Palindromic Repeats sequences associated with Cellular apoptosis susceptibility proteins.Citation
High hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii 2016, 6:27289 Scientific ReportsSponsors
The authors would like to thank Didier Chevret for performing the proteomic analysis and Mickael Beauverger for help with protein extraction and gel migration. We are indebted to Helen McCombie for her helpful language improvement. This work was supported by the Agence Nationale de la Recherche (ANR-10-BLAN-1725 01-Living deep) and G.M. was supported by a PhD fellowship from the Conseil Régional de Bretagne.Publisher
Springer NatureJournal
Scientific ReportsAdditional Links
http://www.nature.com/articles/srep27289ae974a485f413a2113503eed53cd6c53
10.1038/srep27289