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dc.contributor.authorMandal, Pritam
dc.contributor.authorNoutsi, Bakiza Kamal
dc.contributor.authorChaieb, Saharoui
dc.date.accessioned2016-06-02T12:35:12Z
dc.date.available2016-06-02T12:35:12Z
dc.date.issued2016-06-01
dc.identifier.citationCholesterol Depletion from a Ceramide/Cholesterol Mixed Monolayer: A Brewster Angle Microscope Study 2016, 6:26907 Scientific Reports
dc.identifier.issn2045-2322
dc.identifier.pmid27245215
dc.identifier.doi10.1038/srep26907
dc.identifier.urihttp://hdl.handle.net/10754/611561
dc.description.abstractCholesterol is crucial to the mechanical properties of cell membranes that are important to cells’ behavior. Its depletion from the cell membranes could be dramatic. Among cyclodextrins (CDs), methyl beta cyclodextrin (MβCD) is the most efficient to deplete cholesterol (Chol) from biomembranes. Here, we focus on the depletion of cholesterol from a C16 ceramide/cholesterol (C16-Cer/Chol) mixed monolayer using MβCD. While the removal of cholesterol by MβCD depends on the cholesterol concentration in most mixed lipid monolayers, it does not depend very much on the concentration of cholesterol in C16-Cer/Chol monolayers. The surface pressure decay during depletion were described by a stretched exponential that suggested that the cholesterol molecules are unable to diffuse laterally and behave like static traps for the MβCD molecules. Cholesterol depletion causes morphology changes of domains but these disrupted monolayers domains seem to reform even when cholesterol level was low.
dc.description.sponsorshipWe thank King Abdullah University for Science and Technology (KAUST) for financial support. S.C. Thanks Itamar Procaccia for helpful discussions.
dc.language.isoen
dc.publisherSpringer Nature
dc.relation.urlhttp://www.nature.com/articles/srep26907
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
dc.titleCholesterol Depletion from a Ceramide/Cholesterol Mixed Monolayer: A Brewster Angle Microscope Study
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalScientific Reports
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionLawrence Berkeley National Laboratory, 1 cyclotron road, Mailstop 6R-2100, Berkeley, CA-94720, USA
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personMandal, Pritam
kaust.personNoutsi, Bakiza Kamal
kaust.personChaieb, Saharoui
refterms.dateFOA2018-06-13T12:33:59Z


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