Cholesterol Depletion from a Ceramide/Cholesterol Mixed Monolayer: A Brewster Angle Microscope Study
Type
ArticleKAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Physical Science and Engineering (PSE) Division
Date
2016-06-01Online Publication Date
2016-06-01Print Publication Date
2016-07Permanent link to this record
http://hdl.handle.net/10754/611561
Metadata
Show full item recordAbstract
Cholesterol is crucial to the mechanical properties of cell membranes that are important to cells’ behavior. Its depletion from the cell membranes could be dramatic. Among cyclodextrins (CDs), methyl beta cyclodextrin (MβCD) is the most efficient to deplete cholesterol (Chol) from biomembranes. Here, we focus on the depletion of cholesterol from a C16 ceramide/cholesterol (C16-Cer/Chol) mixed monolayer using MβCD. While the removal of cholesterol by MβCD depends on the cholesterol concentration in most mixed lipid monolayers, it does not depend very much on the concentration of cholesterol in C16-Cer/Chol monolayers. The surface pressure decay during depletion were described by a stretched exponential that suggested that the cholesterol molecules are unable to diffuse laterally and behave like static traps for the MβCD molecules. Cholesterol depletion causes morphology changes of domains but these disrupted monolayers domains seem to reform even when cholesterol level was low.Citation
Cholesterol Depletion from a Ceramide/Cholesterol Mixed Monolayer: A Brewster Angle Microscope Study 2016, 6:26907 Scientific ReportsSponsors
We thank King Abdullah University for Science and Technology (KAUST) for financial support. S.C. Thanks Itamar Procaccia for helpful discussions.Publisher
Springer NatureJournal
Scientific ReportsPubMed ID
27245215Additional Links
http://www.nature.com/articles/srep26907ae974a485f413a2113503eed53cd6c53
10.1038/srep26907
Scopus Count
Related articles
- Methyl-β-cyclodextrins preferentially remove cholesterol from the liquid disordered phase in giant unilamellar vesicles.
- Authors: Sanchez SA, Gunther G, Tricerri MA, Gratton E
- Issue date: 2011 May
- Studies on β-sitosterol and ceramide-induced alterations in the properties of cholesterol/sphingomyelin/ganglioside monolayers.
- Authors: Hąc-Wydro K
- Issue date: 2013 Nov
- X-ray grazing incidence diffraction and Langmuir monolayer studies of the interaction of beta-cyclodextrin with model lipid membranes.
- Authors: Flasiński M, Broniatowski M, Majewski J, Dynarowicz-Łatka P
- Issue date: 2010 Aug 15
- Morphology, compressibility and viscoelasticity of the mixed lipid monolayers in the presence of β-carotene.
- Authors: Dopierała K, Skrzypiec M
- Issue date: 2018 Jul
- Antibody labeling of cholesterol/ceramide ordered domains in cell membranes.
- Authors: Scheffer L, Futerman AH, Addadi L
- Issue date: 2007 Dec 17