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    Cholesterol Depletion from a Ceramide/Cholesterol Mixed Monolayer: A Brewster Angle Microscope Study

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    srep26907.pdf
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    Type
    Article
    Authors
    Mandal, Pritam
    Noutsi, Bakiza Kamal cc
    Chaieb, Saharoui cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Physical Science and Engineering (PSE) Division
    Date
    2016-06-01
    Online Publication Date
    2016-06-01
    Print Publication Date
    2016-07
    Permanent link to this record
    http://hdl.handle.net/10754/611561
    
    Metadata
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    Abstract
    Cholesterol is crucial to the mechanical properties of cell membranes that are important to cells’ behavior. Its depletion from the cell membranes could be dramatic. Among cyclodextrins (CDs), methyl beta cyclodextrin (MβCD) is the most efficient to deplete cholesterol (Chol) from biomembranes. Here, we focus on the depletion of cholesterol from a C16 ceramide/cholesterol (C16-Cer/Chol) mixed monolayer using MβCD. While the removal of cholesterol by MβCD depends on the cholesterol concentration in most mixed lipid monolayers, it does not depend very much on the concentration of cholesterol in C16-Cer/Chol monolayers. The surface pressure decay during depletion were described by a stretched exponential that suggested that the cholesterol molecules are unable to diffuse laterally and behave like static traps for the MβCD molecules. Cholesterol depletion causes morphology changes of domains but these disrupted monolayers domains seem to reform even when cholesterol level was low.
    Citation
    Cholesterol Depletion from a Ceramide/Cholesterol Mixed Monolayer: A Brewster Angle Microscope Study 2016, 6:26907 Scientific Reports
    Sponsors
    We thank King Abdullah University for Science and Technology (KAUST) for financial support. S.C. Thanks Itamar Procaccia for helpful discussions.
    Publisher
    Springer Nature
    Journal
    Scientific Reports
    DOI
    10.1038/srep26907
    PubMed ID
    27245215
    Additional Links
    http://www.nature.com/articles/srep26907
    ae974a485f413a2113503eed53cd6c53
    10.1038/srep26907
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Physical Science and Engineering (PSE) Division

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