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    CRISPR/Cas9-Mediated Immunity to Geminiviruses: Differential Interference and Evasion

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    Type
    Article
    Authors
    Ali, Zahir cc
    Ali, Shawkat cc
    Tashkandi, Manal cc
    Zaidi, Syed Shan-e-Ali
    Mahfouz, Magdy M. cc
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Bioscience Program
    Center for Desert Agriculture
    Laboratory for Genome Engineering
    Plant Science
    Plant Science Program
    Date
    2016-05-26
    Online Publication Date
    2016-05-26
    Print Publication Date
    2016-07
    Permanent link to this record
    http://hdl.handle.net/10754/611199
    
    Metadata
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    Abstract
    The CRISPR/Cas9 system has recently been used to confer molecular immunity against several eukaryotic viruses, including plant DNA geminiviruses. Here, we provide a detailed analysis of the efficiencies of targeting different coding and non-coding sequences in the genomes of multiple geminiviruses. Moreover, we analyze the ability of geminiviruses to evade the CRISPR/Cas9 machinery. Our results demonstrate that the CRISPR/Cas9 machinery can efficiently target coding and non-coding sequences and interfere with various geminiviruses. Furthermore, targeting the coding sequences of different geminiviruses resulted in the generation of viral variants capable of replication and systemic movement. By contrast, targeting the noncoding intergenic region sequences of geminiviruses resulted in interference, but with inefficient recovery of mutated viral variants, which thus limited the generation of variants capable of replication and movement. Taken together, our results indicate that targeting noncoding, intergenic sequences provides viral interference activity and significantly limits the generation of viral variants capable of replication and systemic infection, which is essential for developing durable resistance strategies for long-term virus control.
    Citation
    CRISPR/Cas9-Mediated Immunity to Geminiviruses: Differential Interference and Evasion 2016, 6:26912 Scientific Reports
    Sponsors
    We wish to thank members of the Laboratory for Genome Engineering at King Abdullah University of Science and Technology for helpful discussions and comments. The study was supported by King Abdullah University of Science and Technology.
    Publisher
    Springer Nature
    Journal
    Scientific Reports
    DOI
    10.1038/srep26912
    PubMed ID
    27225592
    Additional Links
    http://www.nature.com/articles/srep26912
    ae974a485f413a2113503eed53cd6c53
    10.1038/srep26912
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Plant Science Program; Center for Desert Agriculture

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