CRISPR/Cas9-Mediated Immunity to Geminiviruses: Differential Interference and Evasion
Type
ArticleKAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionBioscience Program
Center for Desert Agriculture
Laboratory for Genome Engineering
Plant Science
Plant Science Program
Date
2016-05-26Online Publication Date
2016-05-26Print Publication Date
2016-07Permanent link to this record
http://hdl.handle.net/10754/611199
Metadata
Show full item recordAbstract
The CRISPR/Cas9 system has recently been used to confer molecular immunity against several eukaryotic viruses, including plant DNA geminiviruses. Here, we provide a detailed analysis of the efficiencies of targeting different coding and non-coding sequences in the genomes of multiple geminiviruses. Moreover, we analyze the ability of geminiviruses to evade the CRISPR/Cas9 machinery. Our results demonstrate that the CRISPR/Cas9 machinery can efficiently target coding and non-coding sequences and interfere with various geminiviruses. Furthermore, targeting the coding sequences of different geminiviruses resulted in the generation of viral variants capable of replication and systemic movement. By contrast, targeting the noncoding intergenic region sequences of geminiviruses resulted in interference, but with inefficient recovery of mutated viral variants, which thus limited the generation of variants capable of replication and movement. Taken together, our results indicate that targeting noncoding, intergenic sequences provides viral interference activity and significantly limits the generation of viral variants capable of replication and systemic infection, which is essential for developing durable resistance strategies for long-term virus control.Citation
CRISPR/Cas9-Mediated Immunity to Geminiviruses: Differential Interference and Evasion 2016, 6:26912 Scientific ReportsSponsors
We wish to thank members of the Laboratory for Genome Engineering at King Abdullah University of Science and Technology for helpful discussions and comments. The study was supported by King Abdullah University of Science and Technology.Publisher
Springer NatureJournal
Scientific ReportsPubMed ID
27225592Additional Links
http://www.nature.com/articles/srep26912ae974a485f413a2113503eed53cd6c53
10.1038/srep26912
Scopus Count
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