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Type
ArticleKAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Computational Bioscience Research Center (CBRC)
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Date
2016-05-11Online Publication Date
2016-05-11Print Publication Date
2016-12Permanent link to this record
http://hdl.handle.net/10754/609438
Metadata
Show full item recordAbstract
Background Cyclic nucleotides have been shown to play important signaling roles in many physiological processes in plants including photosynthesis and defence. Despite this, little is known about cyclic nucleotide-dependent signaling mechanisms in plants since the downstream target proteins remain unknown. This is largely due to the fact that bioinformatics searches fail to identify plant homologs of protein kinases and phosphodiesterases that are the main targets of cyclic nucleotides in animals. Methods An affinity purification technique was used to identify cyclic nucleotide binding proteins in Arabidopsis thaliana. The identified proteins were subjected to a computational analysis that included a sequence, transcriptional co-expression and functional annotation analysis in order to assess their potential role in plant cyclic nucleotide signaling. Results A total of twelve cyclic nucleotide binding proteins were identified experimentally including key enzymes in the Calvin cycle and photorespiration pathway. Importantly, eight of the twelve proteins were shown to contain putative cyclic nucleotide binding domains. Moreover, the identified proteins are post-translationally modified by nitric oxide, transcriptionally co-expressed and annotated to function in hydrogen peroxide signaling and the defence response. The activity of one of these proteins, GLYGOLATE OXIDASE 1, a photorespiratory enzyme that produces hydrogen peroxide in response to Pseudomonas, was shown to be repressed by a combination of cGMP and nitric oxide treatment. Conclusions We propose that the identified proteins function together as points of cross-talk between cyclic nucleotide, nitric oxide and reactive oxygen species signaling during the defence response.Citation
The arabidopsis cyclic nucleotide interactome 2016, 14 (1) Cell Communication and SignalingSponsors
This research was funded by King Abdullah University of Science and Technology.Publisher
Springer NatureJournal
Cell Communication and SignalingPubMed ID
27170143Relations
Is Supplemented By:- [Dataset]
Donaldson, L., Meier, S., & Gehring, C. (2016). The arabidopsis cyclic nucleotide interactome. Figshare. https://doi.org/10.6084/m9.figshare.c.3621143. DOI: 10.6084/m9.figshare.c.3621143 HANDLE: 10754/624135
ae974a485f413a2113503eed53cd6c53
10.1186/s12964-016-0133-2
Scopus Count
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