Show simple item record

dc.contributor.advisorGojobori, Takashi
dc.contributor.authorFatani, Siham
dc.date.accessioned2016-05-12T07:04:24Z
dc.date.available2017-05-12T00:00:00Z
dc.date.issued2016-05
dc.identifier.citationFatani, S. (2016). Investigation and Isolation of Cellulase-Producing microorganisms in the Red Sea. KAUST Research Repository. https://doi.org/10.25781/KAUST-2IM45
dc.identifier.doi10.25781/KAUST-2IM45
dc.identifier.urihttp://hdl.handle.net/10754/609149
dc.description.abstractCellulolytic microorganisms are considered to be key players in biorefinery, especially for the utilization of plant biomass. These organisms have been isolated from various environments. The Red Sea is one of the seas with high biodiversity and a unique environment, characterized by high water temperature and high salinity . However, there is little information regarding cellulases in Red Sea environments. The aim of the present study is to evaluate the Red Sea as a gene resource for microbial cellulase. I first surveyed microbial cellulases in the Red Sea using a method called metagenomes, and then investigated their abundance and diversity. My survey revealed that the Red Sea biome has a substantial abundance and a wide range of cellulase enzymes with substantial abundance, when compared with those in other environments. Next, I tried to isolate cellulase-active microorganisms from the Red Sea and I successfully obtained seven strains of four different taxonomic groups. These strains showed a similarity of 99% identity to Aspergillus ustus, 99% to Staphylococcus pasteuri, 99% to Bacillus aerius and 99% to Bacillus subtilis. The enzyme assay I conducted, revealed that these strains actually secreted active cellulases. These results suggest that the Red Sea environment can be, indeed, an excellent gene resource of microbial cellulases.
dc.language.isoen
dc.subjectRed Sea
dc.subjectMetagenome
dc.subjectCellulases
dc.subjectCellulose
dc.subjectScreening
dc.titleInvestigation and Isolation of Cellulase-Producing microorganisms in the Red Sea
dc.typeThesis
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.rights.embargodate2017-05-12
thesis.degree.grantorKing Abdullah University of Science and Technology
dc.contributor.committeememberArold, Stefan T.
dc.contributor.committeememberMineta, Katsuhiko
thesis.degree.disciplineBioscience
thesis.degree.nameMaster of Science
dc.rights.accessrightsAt the time of archiving, the student author of this thesis opted to temporarily restrict access to it. The full text of this thesis became available to the public after the expiration of the embargo on 2017-05-12.
refterms.dateFOA2017-05-12T00:00:00Z


Files in this item

Thumbnail
Name:
Siham Fatani Thesis.pdf
Size:
794.0Kb
Format:
PDF
Description:
Siham Fatani Thesis

This item appears in the following Collection(s)

Show simple item record