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    Investigation and Isolation of Cellulase-Producing microorganisms in the Red Sea

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    Siham Fatani Thesis.pdf
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    Description:
    Siham Fatani Thesis
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    Type
    Thesis
    Authors
    Fatani, Siham cc
    Advisors
    Gojobori, Takashi cc
    Committee members
    Arold, Stefan T. cc
    Mineta, Katsuhiko cc
    Program
    Bioscience
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Date
    2016-05
    Embargo End Date
    2017-05-12
    Permanent link to this record
    http://hdl.handle.net/10754/609149
    
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    Show full item record
    Access Restrictions
    At the time of archiving, the student author of this thesis opted to temporarily restrict access to it. The full text of this thesis became available to the public after the expiration of the embargo on 2017-05-12.
    Abstract
    Cellulolytic microorganisms are considered to be key players in biorefinery, especially for the utilization of plant biomass. These organisms have been isolated from various environments. The Red Sea is one of the seas with high biodiversity and a unique environment, characterized by high water temperature and high salinity . However, there is little information regarding cellulases in Red Sea environments. The aim of the present study is to evaluate the Red Sea as a gene resource for microbial cellulase. I first surveyed microbial cellulases in the Red Sea using a method called metagenomes, and then investigated their abundance and diversity. My survey revealed that the Red Sea biome has a substantial abundance and a wide range of cellulase enzymes with substantial abundance, when compared with those in other environments. Next, I tried to isolate cellulase-active microorganisms from the Red Sea and I successfully obtained seven strains of four different taxonomic groups. These strains showed a similarity of 99% identity to Aspergillus ustus, 99% to Staphylococcus pasteuri, 99% to Bacillus aerius and 99% to Bacillus subtilis. The enzyme assay I conducted, revealed that these strains actually secreted active cellulases. These results suggest that the Red Sea environment can be, indeed, an excellent gene resource of microbial cellulases.
    Citation
    Fatani, S. (2016). Investigation and Isolation of Cellulase-Producing microorganisms in the Red Sea. KAUST Research Repository. https://doi.org/10.25781/KAUST-2IM45
    DOI
    10.25781/KAUST-2IM45
    ae974a485f413a2113503eed53cd6c53
    10.25781/KAUST-2IM45
    Scopus Count
    Collections
    Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Bioscience Program; MS Theses

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