Cell Surface Enzymatic Engineering-Based Approaches to Improve Cellular Therapies
Type
Book ChapterKAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Date
2014-06-07Online Publication Date
2014-06-07Print Publication Date
2014Permanent link to this record
http://hdl.handle.net/10754/608652
Metadata
Show full item recordAbstract
The cell surface represents the interface between the cell and its environment. As such, the cell surface controls cell–cell interactions and functions such as adhesion and migration, and will transfer external cues to regulate processes such as survival, death, and differentiation. Redefining the cell surface by temporarily (or permanently) modifying the molecular landscape of the plasma membrane affects the way in which the cell interacts with its environment and influences the information that is relayed into the cell along downstream signaling pathways. This chapter outlines the role of key enzymes, the glycosyltransferases, in posttranslationally modifying proteins and lipids to fine-tune cells, ability to migrate. These enzymes are critical in controlling the formation of a platform structure, sialyl Lewis x (sLex), on circulating cells that plays a central role in the recognition and recruitment by selectin counter receptors on endothelial cells that line blood vessels of tissues throughout the body. By developing methods to manipulate the activity of these enzymes and hence the cell surface structures that result, treatments can be envisioned that direct the migration of therapeutic cells to specific locations throughout the body and also to inhibit metastasis of detrimental cells such as circulating tumor cells.Publisher
Elsevier BVISBN
978-1-4557-3146-6ae974a485f413a2113503eed53cd6c53
10.1016/B978-1-4557-3146-6.00009-X