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dc.contributor.authorOthman, Basmah A.
dc.contributor.authorGreenwood, Christina
dc.contributor.authorAbuElela, Ayman
dc.contributor.authorBharath, Anil A.
dc.contributor.authorChen, Shu
dc.contributor.authorTheodorou, Ioannis
dc.contributor.authorDouglas, Trevor
dc.contributor.authorUchida, Maskai
dc.contributor.authorRyan, Mary
dc.contributor.authorMerzaban, Jasmeen
dc.contributor.authorPorter, Alexandra E.
dc.date.accessioned2016-05-09T08:31:04Z
dc.date.available2016-05-09T08:31:04Z
dc.date.issued2016-04-25
dc.identifier.citationCorrelative Light-Electron Microscopy Shows RGD-Targeted ZnO Nanoparticles Dissolve in the Intracellular Environment of Triple Negative Breast Cancer Cells and Cause Apoptosis with Intratumor Heterogeneity 2016 Advanced Healthcare Materials
dc.identifier.issn21922640
dc.identifier.pmid27111660
dc.identifier.doi10.1002/adhm.201501012
dc.identifier.urihttp://hdl.handle.net/10754/608631
dc.description.abstractZnO nanoparticles (NPs) are reported to show a high degree of cancer cell selectivity with potential use in cancer imaging and therapy. Questions remain about the mode by which the ZnO NPs cause cell death, whether they exert an intra- or extracellular effect, and the resistance among different cancer cell types to ZnO NP exposure. The present study quantifies the variability between the cellular toxicity, dynamics of cellular uptake, and dissolution of bare and RGD (Arg-Gly-Asp)-targeted ZnO NPs by MDA-MB-231 cells. Compared to bare ZnO NPs, RGD-targeting of the ZnO NPs to integrin αvβ3 receptors expressed on MDA-MB-231 cells appears to increase the toxicity of the ZnO NPs to breast cancer cells at lower doses. Confocal microscopy of live MDA-MB-231 cells confirms uptake of both classes of ZnO NPs with a commensurate rise in intracellular Zn2+ concentration prior to cell death. The response of the cells within the population to intracellular Zn2+ is highly heterogeneous. In addition, the results emphasize the utility of dynamic and quantitative imaging in understanding cell uptake and processing of targeted therapeutic ZnO NPs at the cellular level by heterogeneous cancer cell populations, which can be crucial for the development of optimized treatment strategies.
dc.language.isoen
dc.publisherWiley
dc.relation.urlhttp://doi.wiley.com/10.1002/adhm.201501012
dc.relation.urlhttp://spiral.imperial.ac.uk/bitstream/10044/1/30266/2/Accepted%20Preprint%20-%20AHM.pdf
dc.rightsArchived with thanks to Wiley
dc.rightsThis file is an open access version redistributed from: http://spiral.imperial.ac.uk/bitstream/10044/1/30266/2/Accepted%20Preprint%20-%20AHM.pdf
dc.titleCorrelative Light-Electron Microscopy Shows RGD-Targeted ZnO Nanoparticles Dissolve in the Intracellular Environment of Triple Negative Breast Cancer Cells and Cause Apoptosis with Intratumor Heterogeneity
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentCell Migration and Signaling Laboratory
dc.identifier.journalAdvanced Healthcare Materials
dc.rights.embargodate2017-04-25
dc.eprint.versionPost-print
dc.contributor.institutionDepartment of Materials; Imperial College London; Royal School of Mines; Exhibition Road London SW7 2AZ UK
dc.contributor.institutionCell and Molecular Biology Research Laboratory; Faculty of Medical Sciences; Post Graduate Medical Institute; Anglia Ruskin University; Bishop Hall Lane; Chelmsford CM1 1SQ UK
dc.contributor.institutionDepartment of Bioengineering; Imperial College London; Royal School of Mines; Exhibition Road London SW7 2AZ UK
dc.contributor.institutionDepartment of Materials; Imperial College London; Royal School of Mines; Exhibition Road London SW7 2AZ UK
dc.contributor.institutionDepartment of Materials; Imperial College London; Royal School of Mines; Exhibition Road London SW7 2AZ UK
dc.contributor.institutionDepartment of Chemistry; Indiana University; 800 E. Kirkwood Avenue Bloomington IN 47405 USA
dc.contributor.institutionDepartment of Chemistry; Indiana University; 800 E. Kirkwood Avenue Bloomington IN 47405 USA
dc.contributor.institutionDepartment of Materials; Imperial College London; Royal School of Mines; Exhibition Road London SW7 2AZ UK
dc.contributor.institutionDepartment of Materials; Imperial College London; Royal School of Mines; Exhibition Road London SW7 2AZ UK
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personAbuElela, Ayman
kaust.personMerzaban, Jasmeen S.
refterms.dateFOA2020-01-22T13:48:18Z
dc.date.published-online2016-04-25
dc.date.published-print2016-06


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