Diversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea
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AbstractIn the present study, the ecological distribution of marine Actinobacteria isolated from seamount and non-seamount stations in the Tyrrhenian Sea was investigated. A collection of 110 isolates was analyzed by Automated Ribosomal Intergenic Spacer Analysis (ARISA) and 16S rRNA gene sequencing of representatives for each ARISA haplotype (n = 49). Phylogenetic analysis of 16S rRNA sequences showed a wide diversity of marine isolates and clustered the strains into 11 different genera, Janibacter, Rhodococcus, Arthrobacter, Kocuria, Dietzia, Curtobacterium, Micrococcus, Citricoccus, Brevibacterium, Brachybacterium and Nocardioides. Interestingly, Janibacter limosus was the most encountered species particularly in seamounts stations, suggesting that it represents an endemic species of this particular ecosystem. The application of BOX-PCR fingerprinting on J. limosus sub-collection (n = 22), allowed their separation into seven distinct BOX-genotypes suggesting a high intraspecific microdiversity among the collection. Furthermore, by screening the biotechnological potential of selected actinobacterial strains, J. limosus was shown to exhibit the most important biosurfactant activity. Our overall data indicates that Janibacter is a major and active component of seamounts in the Tyrrhenian Sea adapted to low nutrient ecological niche.
CitationDiversity, ecological distribution and biotechnological potential of Actinobacteria inhabiting seamounts and non-seamounts in the Tyrrhenian Sea 2016 Microbiological Research
SponsorsThis work was performed in the frame of the CIESM-SUB programme (CIESM Sur-Un- Bateau Cruise I). Pr. Laura Giuliano, who made possible this multidisciplinary research, Pr. Giorgio Budillon, the Chief Scientist of the expedition, the captains Angelo Barca and crews of the R/V Universitatis, are gratefully acknowledged. We thank Dr. Mirko Magagnini for his help in samples collection. This work was partly supported by the Ministry of High Education and Scientific Research of the Tunisian Republic, Grant LR MBA206 and LR11ES31, and the European Union in the ambit of project ULIXES (FP7-KBBE-2010-4, CP-FP-SICA under grant agreement no. 266473).
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