miRNA Repertoires of Demosponges Stylissa carteri and Xestospongia testudinaria
KAUST DepartmentRed Sea Research Center (RSRC)
Biological and Environmental Sciences and Engineering (BESE) Division
Computational Bioscience Research Center (CBRC)
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Marine Science Program
KAUST Environmental Epigenetics Research Program (KEEP)
MetadataShow full item record
AbstractMicroRNAs (miRNAs) are small regulatory RNAs that are involved in many biological process in eukaryotes. They play a crucial role in modulating genetic expression of their targets, which makes them integral components of transcriptional regulatory networks. As sponges (phylum Porifera) are commonly considered the most basal metazoan, the in-depth capture of miRNAs from these organisms provides additional clues to the evolution of miRNA families in metazoans. Here, we identified the core proteins involved in the biogenesis of miRNAs, and obtained evidence for bona fide miRNA sequences for two marine sponges Stylissa carteri and Xestospongia testudinaria (11 and 19 respectively). Our analysis identified several miRNAs that are conserved amongst demosponges, and revealed that all of the novel miRNAs identified in these two species are specific to the class Demospongiae.
CitationmiRNA Repertoires of Demosponges Stylissa carteri and Xestospongia testudinaria 2016, 11 (2):e0149080 PLOS ONE
SponsorsWe thank Annika Haywood, Feras Lafi and Holly Bream for extracting the RNA used for sequencing, as well as two anonymous reviewers for their invaluable suggestions. This project was supported by the King Abdullah University of Science and Technology (KAUST).
PublisherPublic Library of Science (PLoS)
- The evolution of core proteins involved in microRNA biogenesis.
- Authors: Murphy D, Dancis B, Brown JR
- Issue date: 2008 Mar 25
- Correction: miRNA Repertoires of Demosponges Stylissa carteri and Xestospongia testudinaria.
- Authors: Liew YJ, Ryu T, Aranda M, Ravasi T
- Issue date: 2016
- Conservation and divergence of microRNAs in Populus.
- Authors: Barakat A, Wall PK, Diloreto S, Depamphilis CW, Carlson JE
- Issue date: 2007 Dec 31
- Specificity and transcriptional activity of microbiota associated with low and high microbial abundance sponges from the Red Sea.
- Authors: Moitinho-Silva L, Bayer K, Cannistraci CV, Giles EC, Ryu T, Seridi L, Ravasi T, Hentschel U
- Issue date: 2014 Mar
- MicroRNAs and their isomiRs function cooperatively to target common biological pathways.
- Authors: Cloonan N, Wani S, Xu Q, Gu J, Lea K, Heater S, Barbacioru C, Steptoe AL, Martin HC, Nourbakhsh E, Krishnan K, Gardiner B, Wang X, Nones K, Steen JA, Matigian NA, Wood DL, Kassahn KS, Waddell N, Shepherd J, Lee C, Ichikawa J, McKernan K, Bramlett K, Kuersten S, Grimmond SM
- Issue date: 2011 Dec 30