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dc.contributor.authorDeng, Peigang
dc.contributor.authorChang, Donald C.
dc.contributor.authorLee, Yi-Kuen
dc.contributor.authorZhou, Junwei
dc.contributor.authorLi, Gang
dc.date.accessioned2016-02-28T07:59:59Z
dc.date.available2016-02-28T07:59:59Z
dc.date.issued2011-02
dc.identifier.citationDeng P, Chang DC, Lee Y-K, Zhou J, Li G (2011) DNA transfection of bone marrow mesenchymal stem cells using micro electroporation chips. 2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. Available: http://dx.doi.org/10.1109/nems.2011.6017304.
dc.identifier.isbn9781612847757
dc.identifier.doi10.1109/nems.2011.6017304
dc.identifier.urihttp://hdl.handle.net/10754/600249
dc.description.abstractExperimental study of electroporation of bone marrow mesenchymal stem cells (MSCs) at the single-cell level was carried out on a micro EP chip by using single electric rectangular pulse. The threshold values of the electrode potential and pulse width for gas bubble generation on the micro electrodes due to electrolysis of water were revealed as 4.5 volt and 100 μs, respectively. Quantitative EP study was performed with various electric field strengths for various pulse widths, ranging from 20μs to 15ms. Over 1,000 single-cell EP results were used to construct an EP "phase diagram", which delineates the boundaries for (1) effective EP of MSCs and (2) electric cell lysis of MSCs. Finally, the micro EP chip showed successful transfection of the pEGFP-C1 plasmid into the MSCs by properly choosing the electric parameters from the EP "phase diagram". © 2011 IEEE.
dc.description.sponsorshipThis research is in part on work supported by Award No. SA-C0040/UK-COOI6, made by King Abdullah University of Science and Technology and partially supported by a Hong Kong RGC grant (No. 615907).
dc.publisherInstitute of Electrical and Electronics Engineers (IEEE)
dc.subjectbone marrow mesenchymal stem cell
dc.subjectelectroporation
dc.subjectEP phase diagram
dc.subjectmicro electroporation chip
dc.titleDNA transfection of bone marrow mesenchymal stem cells using micro electroporation chips
dc.typeConference Paper
dc.contributor.departmentOCRF- Special Academic Partnership
dc.identifier.journal2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
dc.conference.date20 February 2011 through 23 February 2011
dc.conference.name6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
dc.conference.locationKaohsiung
dc.contributor.institutionDepartment of Biology, HKUST, Clear Water Bay, Kowloon, Hong Kong
dc.contributor.institutionStem Cell and Regeneration Program, School of Biomedical Sciences, CUHK, Shatin, NT, Hong Kong
kaust.personDeng, Peigang
kaust.personLee, Yi-Kuen
kaust.grant.numberSA-C0040
kaust.grant.numberUK-COOI6
kaust.grant.fundedcenterKAUST-HKUST Micro/Nanofluidic Joint Laboratory


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