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    DNA transfection of bone marrow mesenchymal stem cells using micro electroporation chips

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    Type
    Conference Paper
    Authors
    Deng, Peigang
    Chang, Donald C.
    Lee, Yi-Kuen cc
    Zhou, Junwei
    Li, Gang
    KAUST Department
    OCRF- Special Academic Partnership
    KAUST Grant Number
    SA-C0040
    UK-COOI6
    Date
    2011-02
    Permanent link to this record
    http://hdl.handle.net/10754/600249
    
    Metadata
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    Abstract
    Experimental study of electroporation of bone marrow mesenchymal stem cells (MSCs) at the single-cell level was carried out on a micro EP chip by using single electric rectangular pulse. The threshold values of the electrode potential and pulse width for gas bubble generation on the micro electrodes due to electrolysis of water were revealed as 4.5 volt and 100 μs, respectively. Quantitative EP study was performed with various electric field strengths for various pulse widths, ranging from 20μs to 15ms. Over 1,000 single-cell EP results were used to construct an EP "phase diagram", which delineates the boundaries for (1) effective EP of MSCs and (2) electric cell lysis of MSCs. Finally, the micro EP chip showed successful transfection of the pEGFP-C1 plasmid into the MSCs by properly choosing the electric parameters from the EP "phase diagram". © 2011 IEEE.
    Citation
    Deng P, Chang DC, Lee Y-K, Zhou J, Li G (2011) DNA transfection of bone marrow mesenchymal stem cells using micro electroporation chips. 2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. Available: http://dx.doi.org/10.1109/nems.2011.6017304.
    Sponsors
    This research is in part on work supported by Award No. SA-C0040/UK-COOI6, made by King Abdullah University of Science and Technology and partially supported by a Hong Kong RGC grant (No. 615907).
    Publisher
    Institute of Electrical and Electronics Engineers (IEEE)
    Journal
    2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
    Conference/Event name
    6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
    ISBN
    9781612847757
    DOI
    10.1109/nems.2011.6017304
    ae974a485f413a2113503eed53cd6c53
    10.1109/nems.2011.6017304
    Scopus Count
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