First study on gene expression of cement proteins and potential adhesion-related genes of a membranous-based barnacle as revealed from Next-Generation Sequencing technology
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AbstractThis is the first study applying Next-Generation Sequencing (NGS) technology to survey the kinds, expression location, and pattern of adhesion-related genes in a membranous-based barnacle. A total of 77,528,326 and 59,244,468 raw sequence reads of total RNA were generated from the prosoma and the basis of Tetraclita japonica formosana, respectively. In addition, 55,441 and 67,774 genes were further assembled and analyzed. The combined sequence data from both body parts generates a total of 79,833 genes of which 47.7% were shared. Homologues of barnacle cement proteins - CP-19K, -52K, and -100K - were found and all were dominantly expressed at the basis where the cement gland complex is located. This is the main area where transcripts of cement proteins and other potential adhesion-related genes were detected. The absence of another common barnacle cement protein, CP-20K, in the adult transcriptome suggested a possible life-stage restricted gene function and/or a different mechanism in adhesion between membranous-based and calcareous-based barnacles. © 2013 © 2013 Taylor & Francis.
CitationLin H-C, Wong YH, Tsang LM, Chu KH, Qian P-Y, et al. (2013) First study on gene expression of cement proteins and potential adhesion-related genes of a membranous-based barnacle as revealed from Next-Generation Sequencing technology. Biofouling 30: 169–181. Available: http://dx.doi.org/10.1080/08927014.2013.853051.
SponsorsThe authors would like to thank P.-C. Tsai (Academia Sinica) for her assistance in specimen collection, and Genomics BioSci & Tech Company in Taiwan for assisting in specimen processing and data analyses. The authors would also like to thank the three anonymous reviewers for their constructive comments. This work was supported by Academia Sinica Career Development Award [AS-98-CDA-L15]; and National Science Council [Grant NSC-99-2621-B-001-007-MY3] to BKK Chan.
PublisherInforma UK Limited
CollectionsPublications Acknowledging KAUST Support
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